Anti-6X His tag® antibody [HIS.H8] (ab18184)

Hello,
I am very interested in your anti-his antibodies (ab9108, ab18184, ab5000).
However, I was wondering if you could tell me the dissociation constants
of these antibodies to their eppitopes (of any of the ones mentioned
above). It is necessary for me to know for my research.
Kind regards,
--

ANSWER:

Thank you for your inquiry and your interest in our product.

I am sorry to confirm that we do not test / measure the dissociation constants of our antibodies.

I am sorry that I did not have a positive answer for you on this occasion and hope this information is nevertheless helpful. Please do not hesitate to contact us again with any further questions and have a good weekend.

: What is the return policy if the batch of antibody that we order is not binding appropriately? Thanks

ANSWER:

Thank you for contacting Abcam.

Under our http://www.abcam.com/index.html?pageconfig=abpromise, which is valid for 6 months after delivery, the antibody ab18184 is guaranteed it work in ICC/IF, ELISA, Dot Blot, WB, IP, EMSA . If you were to experience any issues while using this antibody and we cannot resolve that issue, we would be happy to either send a replacement or to process a full refund.

Please let me know if there is anything else I can help you with.

Interested in trying this antibody in IHC on fixed whole larvae.

ANSWER:

Thank you very much for your call today andyourinterest in ab18184. Please see below for information about our testing discount program, and let me know if you have any questions.

Sinceab18184 has not been tested in IHC, you are eligible for a free primary antibody on a future purchase if you buy ab18184 now, test it inIHC and submit feedback to us in the form of an Abreview.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab18184 in IHC. I will then send a discount code. This code must be issued before purchasing ab18184 so please wait for my reply before ordering.

2. Purchase ab18184 either by phone, fax, or online (www.abcam.com).

3. Test it in IHC.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab18184turns out to be unsuitable for IHC, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Dear Tech Support Team,

Our customer wishes to test ab9108 andab18184 in sandwich ELISA. Are you interested in this type of the information for the Abreview promotion (The customer buys certain antibodies and upload Abreview on Abcam's website and then we give 100% credit note for this antibody to buy next antibody)?

If your answer is positive, please issue the discount codes. Is it one discount code or 2 different discount codes for each one of the antibodies?

Thanks in advance for your reply.

Kind Regards,

ANSWER:

Thank you for your enquiry.

I am pleased the customer is interested in our testing discount. However, in this case I have some concerns about whether this experiment would work.

Sandwich ELISA procedures can be difficult to optimise as you need to ensure the antibodies are detecting different epitopes on the target protein so they do not interfere with the other antibody binding. The 6 X His tag epitope is small. Once this is bound to the capture antibody, it is unlikely the detection antibody would also be able to bind to as well, due to steric hindrance. If there is any detectable signal, then I am not sure how accurate this would be if they want to quantify.

Therefore, I am sorry we feel a testing discount would not be appropriate in this case. Both the antibodies are tested and guaranteed in indirect ELISA, if the customer would like to try this.

I am sorry this is not better news for you on this occasion. If you have any further questions, please do not hesitate to contact us.

Phoned because this did not work in Western Blot.

ANSWER:

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.