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Immunocytochemistry/ Immunofluorescence - 6X His tag®, antibody [HIS.H8] (ab18184)
![Immunocytochemistry/ Immunofluorescence - 6X His tag®, antibody [HIS.H8] (ab18184)](/ps/datasheet/images/ab18184_2.jpg)
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Western blot - 6X His tag antibody [HIS.H8] (ab18184)
![Western blot - 6X His tag antibody [HIS.H8] (ab18184)](/ps/datasheet/images/ab18184_3.jpg)
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Western blot - 6X His tag® antibody [HIS.H8] (ab18184)
![Western blot - 6X His tag® antibody [HIS.H8] (ab18184)](/ps/datasheet/images/18/ab18184/6X-His-tag-Primary-antibodies-ab18184-2.jpg)
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Product Name
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6X His tag® antibody [HIS.H8]
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See all 6X His tag antibodies (33)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [HIS.H8] to 6X His tag®
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Immunogen
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6x His synthetic peptide.
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Specificity
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Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.
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Tested applications
(see key)
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ChIP/Chip, Dot, ELISA, ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP/Chip: Use at an assay dependent dilution.
Dot: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
ICC/IF: 1/200.
IP: 1/200.
WB: 1/1000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Depends upon the localization of the parent protein tagged with hexahistidine.
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Research areas
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Tags & Cell Markers >> Epitope Tags >> His Tag
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Relevance
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The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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HIS.H8
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Isotype
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IgG2b
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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HIS-TAG® is a trademark of EMD Biosciences, Inc.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this 6X His tag® antibody [HIS.H8] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for 6X His tag® antibody [HIS.H8] images, references, products related to ab18184 and other tools.
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6X His tag® antibody [HIS.H8] images:
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 Immunocytochemistry/ Immunofluorescence - 6X His tag®, antibody [HIS.H8] (ab18184)
Immunofluorescence (red) with ab18184 on His-tag fusion protein in 293 cells.
Left hand panel: untransfected control
Right hand panel: transfected.
Counterstained with DAPI (blue).
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Western blot - 6X His tag antibody [HIS.H8] (ab18184)
All lanes : 6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilution
Lane 1 : 293 cells, untransfected control
Lane 2 : 293 cells transfected with His-tagged vector
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Western blot - 6X His tag® antibody [HIS.H8] (ab18184)
6X His tag® antibody [HIS.H8] (ab18184) at 1/5000 dilution + Conditioned medium from transfected HEK293 cells which produce recombinant secretory protein with a 6XHis-Tag at 5 µg
Secondary
An HRP-conjugated Horse anti mouse polyclonal at 1/5000 dilution This image is courtesy of an anonymous Abreview
See Abreview
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Immunocytochemistry/ Immunofluorescence - 6X His tag® antibody [HIS.H8] (ab18184)
![Immunocytochemistry/ Immunofluorescence - 6X His tag® antibody [HIS.H8] (ab18184)](/ps/datasheet/images/18/ab18184/6X-His-tag-Primary-antibodies-ab18184-4.jpg)
ab18184 at a 1/1000 dilution staining 6X His tag in transfected human HEK293 cells by Immunocytochemistry/ Immunofluorescence incubated for 12 hours at 22°C. PFA fixed. Permeabilized using 0.1% Triton X-100 in PBS. Blocked using 3% BSA for 1 hour at 22°C. Secondary used at a 1/1000 dilution polyclonal Goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 (green). DAPI staining (blue). This image is courtesy of an anonymous abreview.
See Abreview
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References for 6X His tag® antibody [HIS.H8] (ab18184)
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This product has been used in: (two most recent references)
Han C et al. Human SCAMP5, a novel secretory carrier membrane protein, facilitates calcium-triggered cytokine secretion by interaction with SNARE machinery. J Immunol 182:2986-96 (2009). PubMed: 19234194
Chattopadhyay S & Banerjee AK Phosphoprotein, P of human parainfluenza virus type 3 prevents self-association of RNA-dependent RNA polymerase, L. Virology 383:226-36 (2009). WB. PubMed: 19012944
See all 11 publication references for this product.
If you publish research using ab18184 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to 6X His tag®
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6X His tag® antibody [HIS.H8] - more information
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Customer reviews (feedback) regarding 6X His tag® antibody [HIS.H8] |
Customer FAQs regarding 6X His tag® antibody [HIS.H8] |
Protocols for 6X His tag® antibody [HIS.H8] |
Price and availability of products related to 6X His tag® antibody [HIS.H8] |
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6X His tag® antibody [HIS.H8] - related products:
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6X His tag® antibody [HIS.H8] - other tools:
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Contact Abcam with a Technical Enquiry about 6X His tag® antibody [HIS.H8] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - 6X His tag®, antibody [HIS.H8] (ab18184)](/index.html?datasheet=18184#ab18184_2.jpg)
![Western blot - 6X His tag antibody [HIS.H8] (ab18184)](/index.html?datasheet=18184#ab18184_3.jpg)
![Western blot - 6X His tag® antibody [HIS.H8] (ab18184)](/index.html?datasheet=18184#6X-His-tag-Primary-antibodies-ab18184-2.jpg)
