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Anti-6X His tag® antibody [HIS.H8] (ab18184)

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Overview

Product name

Anti-6X His tag® antibody [HIS.H8]
See all 6X His tag® products (38) ...

Description

Mouse monoclonal [HIS.H8] to 6X His tag®

Specificity

Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.

Tested applications

ICC/IF, ELISA, Dot Blot, WB, IP, EMSAmore details

Immunogen

6x His synthetic peptide.

General notes

HIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

HIS.H8

Isotype

IgG2b

Research areas

Tags & Cell Markers >> Epitope Tags >> His Tag

  • Immunocytochemistry/ Immunofluorescence - 6X His tag®, antibody [HIS.H8] (ab18184)Immunocytochemistry/ Immunofluorescence - 6X His tag®, antibody [HIS.H8] (ab18184) image (enlarge)

  • Western blot - 6X His tag antibody [HIS.H8] (ab18184)Western blot - 6X His tag antibody [HIS.H8] (ab18184) image (enlarge)

  • Western blot - 6X His tag® antibody [HIS.H8] (ab18184)Western blot - 6X His tag® antibody [HIS.H8] (ab18184) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab18184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Cellular localization

Depends upon the localization of the parent protein tagged with hexahistidine.

Alternative names

  • 6 His epitope tag antibody
  • Hexa His tag antibody
  • HHHHHH epitope tag antibody
  • HHHHHH tag antibody
  • His tag antibody
see all

Anti-6X His tag® antibody [HIS.H8] images:

  Immunocytochemistry/ Immunofluorescence - 6X His tag®, antibody [HIS.H8] (ab18184)

Immunocytochemistry/ Immunofluorescence - 6X His tag®, antibody [HIS.H8] (ab18184)

Immunofluorescence (red) with ab18184 on His-tag fusion protein in 293 cells.
Left hand panel: untransfected control
Right hand panel: transfected.
Counterstained with DAPI (blue).

  Western blot - 6X His tag antibody [HIS.H8] (ab18184)

Western blot - 6X His tag antibody [HIS.H8] (ab18184)

All lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilution

Lane 1 : 293 cells, untransfected control
Lane 2 : 293 cells transfected with His-tagged vector

  Western blot - 6X His tag® antibody [HIS.H8] (ab18184)

Western blot - 6X His tag® antibody [HIS.H8] (ab18184)

Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/5000 dilution + Conditioned medium from transfected HEK293 cells which produce recombinant secretory protein with a 6XHis-Tag at 5 µg

Secondary
An HRP-conjugated Horse anti mouse polyclonal at 1/5000 dilution

This image is courtesy of an anonymous Abreview

See Abreview

  Immunocytochemistry/ Immunofluorescence - 6X His tag® antibody [HIS.H8] (ab18184)

Immunocytochemistry/ Immunofluorescence - 6X His tag® antibody [HIS.H8] (ab18184)

ab18184 at a 1/1000 dilution staining 6X His tag in transfected human HEK293 cells by Immunocytochemistry/ Immunofluorescence incubated for 12 hours at 22°C. PFA fixed. Permeabilized using 0.1% Triton X-100 in PBS. Blocked using 3% BSA for 1 hour at 22°C. Secondary used at a 1/1000 dilution polyclonal Goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 (green). DAPI staining (blue).

This image is courtesy of an anonymous abreview.

See Abreview

References for Anti-6X His tag® antibody [HIS.H8] (ab18184)

This product has been referenced in:

  • Cojocaru Met al. Transcription Factor IIS Cooperates with the E3 Ligase UBR5 to Ubiquitinate the CDK9 Subunit of the Positive Transcription Elongation Factor B. J Biol Chem 286:5012-22 (2011).Read more (PubMed: 21127351) »
  • Rieger MEet al. The embryonic transcription cofactor LBH is a direct target of the Wnt signaling pathway in epithelial development and in aggressive basal subtype breast cancers. Mol Cell Biol 30:4267-79 (2010). EMSA.Read more (PubMed: 20606007) »

See all 14 publications for this product

Publishing research using ab18184? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"