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Western blot - 6X His tag® antibody (HRP) (ab1187)
(enlarge)
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Product Name
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6X His tag® antibody (HRP)
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See all 6X His tag antibodies (33)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to 6X His tag® (HRP)
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Immunogen
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Synthetic peptide: HHHHHH conjugated to KLH.
BLAST 'HHHHHH' with  or 
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Specificity
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In a direct ELISA against 1ug of 6HIS peptide coupled to BSA as sample, conjugate diluted to 1/40,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000 - 1/20000.
WB: 1/1000 - 1/5000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Depends upon the localization of the parent protein tagged with hexahistidine.
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Research areas
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Tags & Cell Markers >> Epitope Tags >> His Tag
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Relevance
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The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
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6X His tag® antibody (HRP) (ab1187) |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, pH 7 Material safety datasheet (MSDS) for this product:
Thimerosal MSDS
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Purification notes
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Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.
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Form
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Liquid
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Conjugation
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Horse Radish Peroxidase
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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Notes
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HIS-TAG® is a trademark of EMD Biosciences, Inc.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this 6X His tag® antibody (HRP) is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for 6X His tag® antibody (HRP) images, references, products related to ab1187 and other tools.
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6X His tag® antibody (HRP) images:
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 Western blot - 6X His tag® antibody (HRP) (ab1187)
All lanes : 6X His tag® antibody (HRP) (ab1187) at 1/4000 dilution
Lane 1 : 4ug Human His-tagged protein purified from E.coli
Lane 2 : 2ug Human His-tagged protein purified from E.coli
Lane 3 : 1ug Human His-tagged protein purified from E.coli
Lane 4 : 0.5ug Human His-tagged protein purified from E.coli
Observed band size : 136 kDa (why is the actual band size different from the predicted?)
This image is courtesy of an Abreview submitted by Mr Ohrt Thomas
See Abreview
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References for 6X His tag® antibody (HRP) (ab1187)
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This product has been used in: (two most recent references)
Wei H et al. Targeted delivery of tumor antigens to activated dendritic cells via CD11c molecules induces potent antitumor immunity in mice. Clin Cancer Res 15:4612-21 (2009). WB. PubMed: 19584156
O'Malley MA et al. Progress toward heterologous expression of active G-protein-coupled receptors in Saccharomyces cerevisiae: Linking cellular stress response with translocation and trafficking. Protein Sci 18:2356-70 (2009). WB. PubMed: 19760666
See all 6 publication references for this product.
If you publish research using ab1187 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to 6X His tag®
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6X His tag® antibody (HRP) - more information
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Customer reviews (feedback) regarding 6X His tag® antibody (HRP) |
Customer FAQs regarding 6X His tag® antibody (HRP) |
Protocols for 6X His tag® antibody (HRP) |
Price and availability of products related to 6X His tag® antibody (HRP) |
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6X His tag® antibody (HRP) - related products:
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6X His tag® antibody (HRP) - other tools:
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Contact Abcam with a Technical Enquiry about 6X His tag® antibody (HRP) |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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