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Anti-6X His tag® antibody (HRP) (ab1187)

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Overview

Product name

Anti-6X His tag® antibody (HRP)
See all 6X His tag® products (38) ...

Description

Rabbit polyclonal to 6X His tag® (HRP)

Conjugation

HRP

Specificity

In a direct ELISA against 1ug of 6HIS peptide coupled to BSA as sample, conjugate diluted to 1/40,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.

Tested applications

ELISA, WBmore details

Immunogen

Synthetic peptide: HHHHHH conjugated to KLH.

HHHHHH

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

General notes

HIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, pH 7

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.

Clonality

Polyclonal

Isotype

IgG

Research areas

Tags & Cell Markers >> Epitope Tags >> His Tag

  • Western blot - 6X His tag® antibody (HRP) (ab1187)Western blot - 6X His tag® antibody (HRP) (ab1187) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab1187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Cellular localization

Depends upon the localization of the parent protein tagged with hexahistidine.

Alternative names

  • 6 His epitope tag antibody
  • Hexa His tag antibody
  • HHHHHH epitope tag antibody
  • HHHHHH tag antibody
  • His tag antibody
see all

Anti-6X His tag® antibody (HRP) images:

  Western blot - 6X His tag® antibody (HRP) (ab1187)

Western blot - 6X His tag® antibody (HRP) (ab1187)

All lanes : Anti-6X His tag® antibody (HRP) (ab1187) at 1/4000 dilution

Lane 1 : 4ug Human His-tagged protein purified from E.coli
Lane 2 : 2ug Human His-tagged protein purified from E.coli
Lane 3 : 1ug Human His-tagged protein purified from E.coli
Lane 4 : 0.5ug Human His-tagged protein purified from E.coli


Observed band size : 136 kDa (why is the actual band size different from the predicted?)

This image is courtesy of an Abreview submitted by Mr Ohrt Thomas

See Abreview

References for Anti-6X His tag® antibody (HRP) (ab1187)

This product has been referenced in:

  • Youn HSet al. PTOV1 antagonizes MED25 in RAR transcriptional activation. Biochem Biophys Res Commun 404:239-44 (2011). WB.Read more (PubMed: 21110951) »
  • Yu XJet al. Tandem Translation Generates a Chaperone for the Salmonella Type III Secretion System Protein SsaQ. J Biol Chem 286:36098-107 (2011). WB.Read more (PubMed: 21878641) »

See all 8 publications for this product

Publishing research using ab1187? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"