You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-6X His tag® antibody (HRP) (ab3553)

Abreviews (1)Q&A (2)

Overview

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage buffer50 mM HEPES pH 7.0, 0.1 M NaCl, 0.2% BSA, 0.01% Thimerosal
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • Myelomaunknown
  • IsotypeIgY
  • Light chain typeunknown
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab3553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use at an assay dependent concentration. 1/1000.
ELISA ELISA: Use at an assay dependent concentration. a 1/10000 dilution gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti-peptide purification.

Target

  • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
      6 His epitope tag antibodyHexa His tag antibodyHHHHHH epitope tag antibody
      HHHHHH tag antibodyHis tag antibody
    see all

References for Anti-6X His tag® antibody (HRP) (ab3553)

ab3553 has not yet been referenced specifically in any publications.

Publishing research using ab3553 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab3553 Submit a Question for ab3553

Displaying 1 - 3 of 3 results for Abreviews and Q&A

10
Votes: Highest First

Inquiry: Dear Sir/Madam, I am looking for a His-tag probe/antibody, which is already conjugated with HRP, for faster detection of His-tagged recombinant proteins. I would like to know if such a product exists in your product line, and if so, can I get a q...

Read More

Thank you for your inquiry.

We have a number of antibodies in our catalog that might be of interest to you. I would like to direct you to the search facility on our website (www.abcam.com).

Please enter the name of the protein into t...

Read More

Western blot abreview for Anti-6X His tag® antibody (HRP)

 Excellent
Abreviews
Application Western blot
Sample Human Recombinant protein (protein produced in mammalian expression system)
Loading amount 1 µg
Specification protein produced in mammalian expression system
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
abreview image
Username

Verified customer

Submitted Nov 29 2007

Customer Question: Does the chicken anti-His tag polyclonal antibody bind to N-terminal His tags?

Thank you for your enquiry. Yes, this product will detect N-terminal HIS tags. Please contact us again if you require further information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"