Overview

  • Product nameAnti-6X His tag® antibody
    See all 6X His tag® primary antibodies
  • Description
    Rabbit polyclonal to 6X His tag®
  • Tested applicationsWB, IPmore details
  • Immunogen

    A synthetic peptide: HHHHHH (6-His) conjugated to KLH.

  • Positive control
    • ab2431 E. coli Positive Control (Escherichia coli) Whole Cell Lysate - expressing 6X His tag protein. His-tagged recombinant proteins.
  • General notesHIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • FormLiquid
  • Storage instructionsStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Storage bufferpH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab137839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000.
IP 1/500.

Target

  • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody images

  • All lanes : Anti-6X His tag® antibody (ab137839) at 1/1000 dilution

    Lane 1 : Non-transformed E. Coli lysate
    Lane 2 : Non-transformed E. Coli lysate, induced
    Lane 3 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
    Lane 4 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced

    Lysates/proteins at 10 µg per lane.
  • All lanes : Anti-6X His tag® antibody (ab137839)

    Lane 1 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
    Lane 2 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced

    Lysates/proteins at 10 µg per lane.
  • His-tagged protein was immunoprecipitated from transfected cells using 8 ug of ab137839. The precipitated His-tagged protein was detected by ab137839 diluted at 1/500.

References for Anti-6X His tag® antibody (ab137839)

ab137839 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"