Overview

  • Product name
    Anti-6X His tag® antibody
    See all 6X His tag® primary antibodies
  • Description
    Rabbit polyclonal to 6X His tag®
  • Tested applications
    Suitable for: WB, IPmore details
  • Immunogen

    A synthetic peptide: HHHHHH (6-His) conjugated to KLH.

  • Positive control
    • ab2431 E. coli Positive Control (Escherichia coli) Whole Cell Lysate - expressing 6X His tag protein. His-tagged recombinant proteins.
  • General notes
    HIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000.
IP 1/500.

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Images

  • All lanes : Anti-6X His tag® antibody (ab137839) at 1/1000 dilution

    Lane 1 : Non-transformed E. Coli lysate
    Lane 2 : Non-transformed E. Coli lysate, induced
    Lane 3 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
    Lane 4 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced

    Lysates/proteins at 10 µg per lane.
  • All lanes : Anti-6X His tag® antibody (ab137839)

    Lane 1 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
    Lane 2 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced

    Lysates/proteins at 10 µg per lane.
  • His-tagged protein was immunoprecipitated from transfected cells using 8 ug of ab137839. The precipitated His-tagged protein was detected by ab137839 diluted at 1/500.

References

This product has been referenced in:
  • Groß F  et al. Copper amine oxidase 8 regulates arginine-dependent nitric oxide production in Arabidopsis thaliana. J Exp Bot 68:2149-2162 (2017). Read more (PubMed: 28383668) »
  • Konitsiotis AD  et al. Topological analysis of Hedgehog acyltransferase, a multipalmitoylated transmembrane protein. J Biol Chem 290:3293-307 (2015). WB . Read more (PubMed: 25505265) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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