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Products:Tags & Cell Markers >> Epitope Tags >> His Tag
Overview
Product name
Anti-6X His tag® antibody
See all 6X His tag® products (38) ...
Description
Chicken polyclonal to 6X His tag®
Specificity
Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:8000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using Goat Anti Chicken IgY (IgG) and TMB as the substrate. Appropriate specificity controls were run.
Tested applications
WB, ELISAmore details
Immunogen
General notes
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
PBS, pH 7.2, sodium azide, 0.1%
Concentration
Concentration information loading...
Purity
IgY fraction
Purification notes
Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
Clonality
Polyclonal
Isotype
unknown
Light chain type
unknown
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
ELISA
Application notes
This antibody was an effective at 1:1000 (1 µg/ml) dilution using an affinity purified goat anti chicken IgY (IgG) HRP.
The Westerns probed with this antibody are very sharp and clear with extremely low background and no non-specific binding.
Target
Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine.
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
References for Anti-6X His tag® antibody (ab9107)
ab9107 has not yet been referenced specifically in any publications.
Publishing research using ab9107? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"