Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab9136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA 1/100 - 1/500.
ICC/IF Use at an assay dependent concentration. PubMed: 22247289
ICC 1/200 - 1/2000.
WB 1/1000 - 1/30000.

Target

  • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody images

  • ab9136, staining 6X His tag® in VMP001-conjugated nanoparticles by Immunocytochemistry/ Immunofluorescence. An AlexaFluor®488-conjugated anti-goat IgG was used as the secondary antibody.

References for Anti-6X His tag® antibody (ab9136)

This product has been referenced in:
  • Moon JJ  et al. Enhancing humoral responses to a malaria antigen with nanoparticle vaccines that expand Tfh cells and promote germinal center induction. Proc Natl Acad Sci U S A 109:1080-5 (2012). ICC/IF . Read more (PubMed: 22247289) »
  • Moon JJ  et al. Antigen-displaying lipid-enveloped PLGA nanoparticles as delivery agents for a Plasmodium vivax malaria vaccine. PLoS One 7:e31472 (2012). ICC/IF . Read more (PubMed: 22328935) »

See all 2 Publications for this product

Product Wall

Here is the answer to your question: I'm afraid the antibody has not been tested for its ability to recognize internal epitopes, sorry about the inconvenience.

Thank you for your enquiry. To our knowledge, these antibodies have yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase one of these products, please let us know how you get...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"