Overview

  • Product nameAnti-6X His tag® antibody [3H2201]
    See all 6X His tag® primary antibodies
  • Description
    Mouse monoclonal [3H2201] to 6X His tag®
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, IP, WBmore details
  • Species reactivity
    It reacts against all His tagged proteins so far tested. In Western blotting of bacterial extracts the antibody has been shown not to react with any endogenous products, although some Reactivity may be seen with extracts of insect or mammalian cells.
  • Immunogen

    PAX6 transcription factor linked to histidine tag.

  • General notesHIS-TAG® is a trademark of EMD Biosciences, Inc.


    ab15149 recognises proteins containing the motif HHHHHH and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesTissue cuture supernatant purified by Protein G affinity chromatography.
  • Primary antibody notesab15149 recognises proteins containing the motif HHHHHH and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells.
  • ClonalityMonoclonal
  • Clone number3H2201
  • MyelomaNS1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/500 - 1/100.

Target

  • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References for Anti-6X His tag® antibody [3H2201] (ab15149)

This product has been referenced in:

See all 4 Publications for this product

Product Wall

Thank you for contacting us.

I can confirm that all four products, the ab5000, ab15149, ab18184 and ab81663 detect proteins, which contain either an N or C-terminal HHHHHHGS (6x) epitope tag.

Important aspects for his tag recognition ...

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Thank you for contacting us and your interest in our products.

The request for a quote for the antibodies mentioned has been passed on to one of my colleagues who will get back to you shortly with this information. In regards to your other qu...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. Even though ab15149 has not been tested in ELISA, since the previous lot worked okay we will offer a replace...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire and I have a few comments. Please can you clarify how you have been applying this antibody and the...

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Thank you for your enquiry. Since you're looking for a 6X His tag antibody that works in both Western blotting and IHC, I would recommend ab15149. This antibody has been tested for application in Western blotting and Immunohistochemistry (Frozen sectio...

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Thank you for your enquiry and your interest in our products. This antibody recognises proteins containing the motif HHHHHH (5x His) and it is therefore of value in detecting proteins containing histidine tags (either at the N- or C-terminal). The a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"