Overview

  • Product nameAnti-6X His tag® antibody
    See all 6X His tag® primary antibodies
  • Description
    Rabbit polyclonal to 6X His tag®
  • SpecificityThis antibody recognizes His tagged fusion proteins. It is a tag antibody.
  • Tested applicationsSuitable for: WB, IP, ELISAmore details
  • Immunogen

    A synthetic peptide of HHHHHH conjugated to a carrier protein.

  • Positive control
    • His tagged fused proteins.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml.
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.

Target

  • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody images

  • All lanes : Anti-6X His tag® antibody (ab125262) at 0.5 µg/ml

    Lane 1 : His tagged recombinant protein (N terminal tag)
    Lane 2 : His tagged recombinant protein (N terminal tag)
    Lane 3 : His tagged recombinant protein (C terminal tag)

References for Anti-6X His tag® antibody (ab125262)

ab125262 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"