The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml.
Use a concentration of 2 - 5 µg/ml.
Use a concentration of 0.01 - 0.1 µg/ml.
RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
6 His epitope tag antibody
Hexa His tag antibody
HHHHHH epitope tag antibody
HHHHHH tag antibody
His tag antibody
Polyhistidine Tag antibody
Anti-6X His tag® antibody images
Western blot - Anti-6X His tag® antibody (ab125262)
All lanes : Anti-6X His tag® antibody (ab125262) at 0.5 µg/ml
Lane 1 : His tagged recombinant protein (N terminal tag) Lane 2 : His tagged recombinant protein (N terminal tag) Lane 3 : His tagged recombinant protein (C terminal tag)
References for Anti-6X His tag® antibody (ab125262)
has not yet been referenced specifically in any publications.
Publishing research using ab125262? Please let us know so that we can cite the reference in this datasheet.
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