Anti-6X His tag® antibody (ab137839)
Key features and details
- Rabbit polyclonal to 6X His tag®
- Suitable for: WB, IP
- Reacts with: Species independent
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-6X His tag® antibody
See all 6X His tag® primary antibodies -
Description
Rabbit polyclonal to 6X His tag® -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
A synthetic peptide: HHHHHH (6-His) conjugated to KLH.
-
Positive control
- ab2431 E. coli Positive Control (Escherichia coli) Whole Cell Lysate - expressing 6X His tag protein. His-tagged recombinant proteins.
-
General notes
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137839 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000.
|
|
IP |
1/500.
|
Notes |
---|
WB
1/1000 - 1/10000. |
IP
1/500. |
Target
-
Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Images
-
All lanes : Anti-6X His tag® antibody (ab137839) at 1/1000 dilution
Lane 1 : Non-transformed E. Coli lysate
Lane 2 : Non-transformed E. Coli lysate, induced
Lane 3 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
Lane 4 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced
Lysates/proteins at 10 µg per lane.
12% SDS PAGE -
All lanes : Anti-6X His tag® antibody (ab137839)
Lane 1 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
Lane 2 : E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced
Lysates/proteins at 10 µg per lane. -
His-tagged protein was immunoprecipitated from transfected cells using 8 ug of ab137839. The precipitated His-tagged protein was detected by ab137839 diluted at 1/500.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (13)
ab137839 has been referenced in 13 publications.
- van Elsas MJ et al. Regulatory T Cell Depletion Using a CRISPR Fc-Optimized CD25 Antibody. Int J Mol Sci 23:N/A (2022). PubMed: 35955841
- West J et al. Neuroserpin and transthyretin are extracellular chaperones that preferentially inhibit amyloid formation. Sci Adv 7:eabf7606 (2021). PubMed: 34890220
- van der Schoot JMS et al. Functional diversification of hybridoma-produced antibodies by CRISPR/HDR genomic engineering. Sci Adv 5:eaaw1822 (2019). PubMed: 31489367
- Shen K et al. Dual Role of Ribosome-Binding Domain of NAC as a Potent Suppressor of Protein Aggregation and Aging-Related Proteinopathies. Mol Cell 74:729-741.e7 (2019). PubMed: 30982745
- Fedorova O et al. Novel isatin-derived molecules activate p53 via interference with Mdm2 to promote apoptosis. Cell Cycle 17:1917-1930 (2018). PubMed: 30109812