• Product nameAnti-6X His tag® antibody
    See all 6X His tag® primary antibodies
  • Description
    Rabbit polyclonal to 6X His tag®
  • SpecificityThe antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Tested applicationsSuitable for: WBmore details
  • Immunogen

    Synthetic peptide 6X His tag® conjugated with a C-terminal cisteine.

    (Peptides available as ab14941 and ab14943.)

  • Positive control
    • E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein, ab2431.
  • General notes

    HIS-TAG® is a trademark of EMD Biosciences, Inc.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab14923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml.


  • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody images

  • Anti-6X His tag® antibody (ab14923) at 0.6 µg/ml + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) at 10 µg

    Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

References for Anti-6X His tag® antibody (ab14923)

This product has been referenced in:
  • Pokidysheva E  et al. Biological role of prolyl 3-hydroxylation in type IV collagen. Proc Natl Acad Sci U S A 111:161-6 (2014). Read more (PubMed: 24368846) »
  • Knapp RT  et al. Hsp70 cochaperones HspBP1 and BAG-1M differentially regulate steroid hormone receptor function. PLoS One 9:e85415 (2014). WB . Read more (PubMed: 24454860) »

See all 3 Publications for this product

Product Wall

I regret to inform you that we do not have this value. This antibody has been tested and characterized for Western blot application only.


Thank you for your enquiry regarding ab14923.

I regret to inform you that we do not perform kinetic studies for primary antibodies since it is not part of our standard QC procedure.


If you need any further assis...

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Vielen Dank für Ihre Antwort.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten.Ich habeeine kostenlose Ersatzlieferung mit einem Röhrchen ab14923für Sie in Auftrag gegeben. Sie hat die Referenznummer xx und...

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Vielen Dank für all diese Informationen. In der Tat sind diese Informationen uns sehr nützlich, um einerseits das Problem zu versuchen zu verstehen, und andererseits auch für unsere Qualitätsreinvestigation.

Es t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 40 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 23 2010

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab14923 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the ...

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Thank you for your enquiry. I think that there still exists the possibility that the antibody might still work well if the blocking buffer is changed. I realize that with the Odyssey system the first protocol to try is using the conditions recommen...

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