• Product nameAnti-6X His tag® antibody
    See all 6X His tag® primary antibodies
  • Description
    Goat polyclonal to 6X His tag®
  • SpecificityThis antibody was verified by ELISA against peptide conjugated to BSA(HHHHHH/BSA).
  • Tested applicationsSuitable for: ELISA, ICC/IF, ICC, WBmore details
  • Immunogen

    Synthetic peptide:


    (6-His) conjugated to KLH.

  • General notesHIS-TAG® is a trademark of EMD Biosciences, Inc.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab9136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100 - 1/500.
ICC/IF Use at an assay dependent concentration. PubMed: 22247289
ICC 1/200 - 1/2000.
WB 1/1000 - 1/30000.


  • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody images

  • ab9136, staining 6X His tag® in VMP001-conjugated nanoparticles by Immunocytochemistry/ Immunofluorescence. An AlexaFluor®488-conjugated anti-goat IgG was used as the secondary antibody.

References for Anti-6X His tag® antibody (ab9136)

This product has been referenced in:
  • Fischer F  et al. Identification of potential mitochondrial CLPXP protease interactors and substrates suggests its central role in energy metabolism. Sci Rep 5:18375 (2015). WB . Read more (PubMed: 26679294) »
  • Moon JJ  et al. Enhancing humoral responses to a malaria antigen with nanoparticle vaccines that expand Tfh cells and promote germinal center induction. Proc Natl Acad Sci U S A 109:1080-5 (2012). ICC/IF . Read more (PubMed: 22247289) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Liver)
Permeabilization No
Specification Liver
Blocking step BSA as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 26 2016

Here is the answer to your question: I'm afraid the antibody has not been tested for its ability to recognize internal epitopes, sorry about the inconvenience.

Thank you for your enquiry. To our knowledge, these antibodies have yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase one of these products, please let us know how you get...

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