Overview

  • Product name
    Anti-6X His tag® antibody
    See all 6X His tag® primary antibodies
  • Description
    Goat polyclonal to 6X His tag®
  • Specificity
    This antibody was verified by ELISA against peptide conjugated to BSA(HHHHHH/BSA).
  • Tested applications
    Suitable for: ELISA, ICC/IF, ICC, WBmore details
  • Immunogen

    Synthetic peptide:

    HHHHHH

    (6-His) conjugated to KLH.

  • General notes
    HIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100 - 1/500.
ICC/IF Use at an assay dependent concentration. PubMed: 22247289
ICC 1/200 - 1/2000.
WB 1/1000 - 1/30000.

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody images

  • ab9136, staining 6X His tag® in VMP001-conjugated nanoparticles by Immunocytochemistry/ Immunofluorescence. An AlexaFluor®488-conjugated anti-goat IgG was used as the secondary antibody.

References for Anti-6X His tag® antibody (ab9136)

This product has been referenced in:
  • Fischer F  et al. Identification of potential mitochondrial CLPXP protease interactors and substrates suggests its central role in energy metabolism. Sci Rep 5:18375 (2015). WB . Read more (PubMed: 26679294) »
  • Fischer F  et al. RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model. Sci Rep 5:12697 (2015). Read more (PubMed: 26220011) »

See all 4 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 26 2016

I'm afraid the antibody has not been tested for its ability to recognize internal epitopes, sorry about the inconvenience.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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