Anti-6X His tag® antibody [AD1.1.10] (ab81663)

Overview

  • Product nameAnti-6X His tag® antibody [AD1.1.10]
    See all 6X His tag® primary antibodies
  • Description
    Mouse monoclonal [AD1.1.10] to 6X His tag®
  • SpecificityThis antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. It reacts against all His tagged proteins so far tested.
  • Tested applicationsSuitable for: WB, IP, ELISAmore details
  • Immunogen

    PAX6 transcription factor linked to histidine tag.

  • General notesHIS-TAG® is a trademark of EMD Biosciences, Inc.


    The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. In Western blotting of bacterial extracts clone ADI.I.10 has been shown not to cross react with any endogenous products, although some cross reactivity may be seen with extracts of insect or mammalian cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesThe antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. In Western blotting of bacterial extracts clone ADI.I.10 has been shown not to cross react with any endogenous products, although some cross reactivity may be seen with extracts of insect or mammalian cells.
  • ClonalityMonoclonal
  • Clone numberAD1.1.10
  • MyelomaNS1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References for Anti-6X His tag® antibody [AD1.1.10] (ab81663)

ab81663 has not yet been referenced specifically in any publications.

Product Wall

According to our records, ab125262 was proving difficult to use inELISA and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better underst...

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Thank you for contacting us.

I can confirm that all four products, the ab5000, ab15149, ab18184 and ab81663 detect proteins, which contain either an N or C-terminal HHHHHHGS (6x) epitope tag.

Important aspects for his tag recognition ...

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Thank you for your email. I am sorry to hear that you have been having trouble using antibody ab12562.

I have checked the information you have kindly provided and have few more question in order to understand the problem better;

- W...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. Even though ab15149 has not been tested in ELISA, since the previous lot worked okay we will offer a replace...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"