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PAX6 transcription factor linked to histidine tag.
The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. In Western blotting of bacterial extracts clone ADI.I.10 has been shown not to cross react with any endogenous products, although some cross reactivity may be seen with extracts of insect or mammalian cells.
Our Abpromise guarantee covers the use of ab81663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml.|
|IP||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|