Anti-6X His tag® antibody [AD1.1.10] (Alkaline Phosphatase) (ab81652)

Overview

  • Product name
    Anti-6X His tag® antibody [AD1.1.10] (Alkaline Phosphatase)
    See all 6X His tag® primary antibodies
  • Description
    Mouse monoclonal [AD1.1.10] to 6X His tag® (Alkaline Phosphatase)
  • Conjugation
    Alkaline Phosphatase
  • Specificity
    ab81652 detects polyhistidine tagged proteins with the 6X His tag® located at either the amino- or carboxyterminus.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    ab81652 recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags.
  • Immunogen

    PAX6 transcription factor linked to 6X His tag®

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 2mM Sodium Azide
    Constituents: 25mM Tris, 150mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    AD1.1.10
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000.

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References

ab81652 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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