Anti-6X His tag® antibody [AD1.1.10] (Alkaline Phosphatase) (ab81652)

Overview

  • Product nameAnti-6X His tag® antibody [AD1.1.10] (Alkaline Phosphatase)
    See all 6X His tag® primary antibodies
  • Description
    Mouse monoclonal [AD1.1.10] to 6X His tag® (Alkaline Phosphatase)
  • ConjugationAlkaline Phosphatase
  • Specificityab81652 detects polyhistidine tagged proteins with the 6X His tag® located at either the amino- or carboxyterminus.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    ab81652 recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags.
  • Immunogen

    PAX6 transcription factor linked to 6X His tag®

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 2mM Sodium Azide
    Constituents: 25mM Tris, 150mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • ClonalityMonoclonal
  • Clone numberAD1.1.10
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000.

Target

  • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References for Anti-6X His tag® antibody [AD1.1.10] (Alkaline Phosphatase) (ab81652)

ab81652 has not yet been referenced specifically in any publications.

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Thank you for contacting us. We do not offer test or trial size samples, however our Abpromise® guarantee ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund. ...

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Thank you for contacting us today. I can confirm that ab81652 is provided in 1ml of water. Please let me know if there is anything else I can help you with.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"