Anti-6X His tag® antibody [AD1.1.10] (Allophycocyanin) (ab72579)

Overview

  • Product nameAnti-6X His tag® antibody [AD1.1.10] (Allophycocyanin)
    See all 6X His tag® primary antibodies
  • Description
    Mouse monoclonal [AD1.1.10] to 6X His tag® (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • Specificityab72579 detects polyhistidine tags located at either the amino- or carboxyterminus.
  • Tested applicationsSuitable for: Flow Cyt, FRETmore details
  • Species reactivity
    Not applicable
  • Immunogen

    PAX6 transcription factor linked to 6X His tag®

  • General notes

    Excitation max. 652 nm. Emission max. 657.5 nm. F/P 1:1.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 2mM Sodium Azide
    Constituents: 0.1% BSA, 50mM Sucrose, 150mM Sodium chloride, 100mM Sodium phosphate, pH 7.4
  • Concentration information loading...
  • PuritySize exclusion
  • Purification notesSize exclusion chromatography as well as some proprietary methods for purifying the final materials were used.
  • ClonalityMonoclonal
  • Clone numberAD1.1.10
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab37391-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FRET Use at an assay dependent concentration.

Target

  • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References for Anti-6X His tag® antibody [AD1.1.10] (Allophycocyanin) (ab72579)

ab72579 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"