Anti-6X His tag® antibody [AD1.1.10] (Biotin) (ab15145)

Overview

  • Product name
    Anti-6X His tag® antibody [AD1.1.10] (Biotin)
    See all 6X His tag® primary antibodies
  • Description
    Mouse monoclonal [AD1.1.10] to 6X His tag® (Biotin)
  • Conjugation
    Biotin
  • Specificity
    The clone number has been updated from (3H2201) to (AD1.1.10) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    In Western Blot of bacterial extracts, the antibody has been shown not to crossreact with any endogenous products. Some Reactivity may be seen with extracts of insect or mammalian cells.
  • Immunogen

    His-His-His-His-His-His conjugated to KLH.

  • General notes
    HIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    AD1.1.10
  • Myeloma
    NS1
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
ELISA 1/500 - 1/1000.

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References

ab15145 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

The biotin binding of ab15145 has not been quantified, but it is typically in the range of 4-6 biotin molecules / antibody. The biotin is linked through lysine residues on the antibody surface.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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