Anti-6X His tag® antibody [AD1.1.10] (DyLight® 488) (ab117512)

Overview

  • Product name
    Anti-6X His tag® antibody [AD1.1.10] (DyLight® 488)
    See all 6X His tag® primary antibodies
  • Description
    Mouse monoclonal [AD1.1.10] to 6X His tag® (DyLight® 488)
  • Conjugation
    DyLight® 488. Ex: 493nm, Em: 518nm
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-Frmore details
  • Immunogen

    Synthetic peptide of 6X His tag®.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 8.20
    Constituent: 99% Tris buffered saline
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    AD1.1.10
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/5000.
Flow Cyt Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 25285446

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References

This product has been referenced in:
  • Imhof BA  et al. CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation. Proc Natl Acad Sci U S A 113:E4847-56 (2016). Read more (PubMed: 27482114) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab117512.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up