The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/5000.
Use at an assay dependent concentration.
RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
6 His epitope tag antibody
Hexa His tag antibody
HHHHHH epitope tag antibody
HHHHHH tag antibody
His tag antibody
Polyhistidine Tag antibody
References for Anti-6X His tag® antibody [AD1.1.10] (DyLight® 550) (ab117507)
has not yet been referenced specifically in any publications.
Publishing research using ab117507? Please let us know so that we can cite the reference in this datasheet.
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