DyLight® 550 Anti-6X His tag® antibody [AD1.1.10] (ab117507)
Key features and details
- DyLight® 550 Mouse monoclonal [AD1.1.10] to 6X His tag®
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Species independent
- Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
DyLight® 550 Anti-6X His tag® antibody [AD1.1.10]
See all 6X His tag® primary antibodies -
Description
DyLight® 550 Mouse monoclonal [AD1.1.10] to 6X His tag® -
Host species
Mouse -
Conjugation
DyLight® 550. Ex: 562nm, Em: 576nm -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to 6X His tag®.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months. -
Storage buffer
pH: 7.40
Preservative: 0.013% Sodium azide
Constituents: 0.58% Sodium chloride, 95% Sodium phosphate -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
AD1.1.10 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- PerCP Anti-6X His tag® antibody [AD1.1.10] (ab117496)
- DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)
- DyLight® 488 Anti-6X His tag® antibody [AD1.1.10] (ab117512)
- Biotin Anti-6X His tag® antibody [AD1.1.10] (ab15145)
- Anti-6X His tag® antibody [AD1.1.10] (ab15149)
- HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563)
- Europium Anti-6X His tag® antibody [AD1.1.10] (ab187912)
- PE Anti-6X His tag® antibody [AD1.1.10] (ab72467)
- SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab72579)
- Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (ab81652)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/500 - 1/5000.
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|
Flow Cyt |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
1/500 - 1/5000. |
Flow Cyt
Use at an assay dependent concentration. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab117507 has been referenced in 1 publication.
- Prasai B et al. The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells. Nat Commun 12:3970 (2021). PubMed: 34172739