Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650) (ab117504)

Overview

  • Product name
    Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650)
    See all 6X His tag® primary antibodies
  • Description
    Mouse monoclonal [AD1.1.10] to 6X His tag® (DyLight® 650)
  • Conjugation
    DyLight® 650. Ex: 654nm, Em: 673nm
  • Tested applications
    Suitable for: ICC/IF, Flow Cytmore details
  • Immunogen

    Synthetic peptide corresonding to 6X His tag®.

  • General notes
    Stable for 1yr.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 8.20
    Constituent: 99% Tris buffered saline
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    AD1.1.10
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/5000.
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650) images

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells transfected with His-tagged actin labeling 6X His tag® with ab117504 at 1/1000 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X100 in PBS. The cells were blocked with 5% BSA for 12 hours at 4°C, followed by incubation with Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650) (ab117504) for 2 hours at 25°C.

    See Abreview

References for Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650) (ab117504)

This product has been referenced in:
  • Imhof BA  et al. CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation. Proc Natl Acad Sci U S A 113:E4847-56 (2016). Read more (PubMed: 27482114) »

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells transfected with His-tagged actin)
Permeabilization
Yes - 0.1% Triton-X100 in PBS
Specification
HeLa cells transfected with His-tagged actin
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
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Submitted Oct 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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