Anti-6X His tag® antibody (Agarose) (ab1231)

Overview

  • Product nameAnti-6X His tag® antibody (Agarose)
    See all 6X His tag® primary antibodies
  • Description
    Goat polyclonal to 6X His tag® (Agarose)
  • ConjugationAgarose
  • Tested applicationsSuitable for: IPmore details
    Unsuitable for: ChIP
  • Immunogen

    Full length native

    HHHHHH

    (6-His)(purified): conjugated to KLH.

  • General notesHIS-TAG® is a trademark of EMD Biosciences, Inc.

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

Properties

Applications

Our Abpromise guarantee covers the use of ab1231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Use 20-40 µl of gel slurry per 0.1-1 mg of protein lysate or extract.
  • Application notesIs unsuitable for ChIP.
  • Target

    • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
    • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
    • Alternative names
      • 6 His epitope tag antibody
      • Hexa His tag antibody
      • HHHHHH epitope tag antibody
      • HHHHHH tag antibody
      • His tag antibody
      • Polyhistidine Tag antibody
      see all

    References for Anti-6X His tag® antibody (Agarose) (ab1231)

    ab1231 has not yet been referenced specifically in any publications.

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    Vielen Dank fürIhre Anfrage.

    Das Labor hat bestätig,dass in 400ul Puffer (50% Slurry)es 200ul Beads hat(und 100ugAntikörper).

    Hier ist ein Link zu den Beads, welchebenutzt wurden. Ich bin mir nicht sicher, wie Sie ge...

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    The type of agarose beads that were used were - Uniflow 4 from Sterogene. If you have any additional questions, please contact us again.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"