Anti-6X His tag® antibody (Agarose) (ab90663)

Overview

  • Product name
    Anti-6X His tag® antibody (Agarose)
    See all 6X His tag® primary antibodies
  • Description
    Rabbit polyclonal to 6X His tag® (Agarose)
  • Conjugation
    Agarose
  • Tested applications
    Suitable for: IPmore details
  • Immunogen

    6XHis tag (HHHHHH) conjugated to KLH.

  • General notes
    Amount: 0.1 mg antibody/200 µl of gel.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab90663 was affinity purified using the peptide immobilized on solid support and coupled to agarose beads using a cyanogen bromide method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
  • Application notes
    IP: Use 15-25 µl of gel slurry per 0.1 to 1 mg of protein lysate or extract.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
    • Cellular localization
      Depends upon the localization of the parent protein tagged with hexahistidine.
    • Alternative names
      • 6 His epitope tag antibody
      • Hexa His tag antibody
      • HHHHHH epitope tag antibody
      • HHHHHH tag antibody
      • His tag antibody
      • Polyhistidine Tag antibody
      see all

    References

    ab90663 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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