Anti-6X His tag® antibody (Agarose) (ab90663)

Overview

  • Product nameAnti-6X His tag® antibody (Agarose)
    See all 6X His tag® primary antibodies
  • Description
    Rabbit polyclonal to 6X His tag® (Agarose)
  • ConjugationAgarose
  • Tested applicationsSuitable for: IPmore details
  • Immunogen

    6XHis tag (HHHHHH) conjugated to KLH.

  • General notesAmount: 0.1 mg antibody/200 µl of gel.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab90663 was affinity purified using the peptide immobilized on solid support and coupled to agarose beads using a cyanogen bromide method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
  • Application notesIP: Use 15-25 µl of gel slurry per 0.1 to 1 mg of protein lysate or extract.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
    • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
    • Alternative names
      • 6 His epitope tag antibody
      • Hexa His tag antibody
      • HHHHHH epitope tag antibody
      • HHHHHH tag antibody
      • His tag antibody
      • Polyhistidine Tag antibody
      see all

    References for Anti-6X His tag® antibody (Agarose) (ab90663)

    ab90663 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"