Anti-6X His tag® antibody (Biotin) (ab27025)

Overview

  • Product nameAnti-6X His tag® antibody (Biotin)
    See all 6X His tag® primary antibodies
  • Description
    Rabbit polyclonal to 6X His tag® (Biotin)
  • ConjugationBiotin
  • SpecificityThis antibody recognises 6x His tag.
  • Tested applicationsSuitable for: Flow Cyt, WB, ELISAmore details
  • Immunogen

    HHHHHH conjugated with KLH.

  • General notesHIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.2% BSA, PBS, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was purified using the peptide immobilized on a solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab200208 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/1000 - 1/5000.
ELISA 1/2000 - 1/10000.

Target

  • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References for Anti-6X His tag® antibody (Biotin) (ab27025)

This product has been referenced in:
  • Spång HC  et al. Heterodimeric Barnase-Barstar Vaccine Molecules: Influence of One versus Two Targeting Units Specific for Antigen Presenting Cells. PLoS One 7:e45393 (2012). WB . Read more (PubMed: 23028981) »
  • Lee HK  et al. In vivo requirement for Atg5 in antigen presentation by dendritic cells. Immunity 32:227-39 (2010). Read more (PubMed: 20171125) »

See all 2 Publications for this product

Product Wall

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Purified protein (recombinant human fibronectin domain)
Loading amount 0.1 µg
Specification recombinant human fibronectin domain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Sep 04 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Saccharomyces cerevisiae Cell (yeast expressing a his tagged scFv)
Specification yeast expressing a his tagged scFv
Preparation Cell harvesting/tissue preparation method: yeast washed once in PBS
Sample buffer: PBS
Fixation none
Permeabilization No
Gating Strategy sample was gated to include all yeast cells
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Abcam user community

Verified customer

Submitted Jun 24 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Samples pre and post enzymatic his tag cleavage)
Loading amount 4 µg
Specification Samples pre and post enzymatic his tag cleavage
Treatment His tag purified supernatant followed by a reaction to cleave the his tag
Gel Running Conditions Non-reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jun 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"