The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ab200208 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
1/1000 - 1/5000.
1/2000 - 1/10000.
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Depends upon the localization of the parent protein tagged with hexahistidine.
References for Anti-6X His tag® antibody (Biotin) (ab27025)
This product has been referenced in:
Spång HC et al. Heterodimeric Barnase-Barstar Vaccine Molecules: Influence of One versus Two Targeting Units Specific for Antigen Presenting Cells. PLoS One7:e45393 (2012).
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