Overview

  • Product name
    Anti-6X His tag® antibody (Biotin)
    See all 6X His tag® primary antibodies
  • Description
    Rabbit polyclonal to 6X His tag® (Biotin)
  • Conjugation
    Biotin
  • Specificity
    This antibody recognises 6x His tag.
  • Tested applications
    Suitable for: Flow Cyt, WB, ELISAmore details
  • Immunogen

    HHHHHH conjugated with KLH.

  • General notes
    HIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.2% BSA, PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was purified using the peptide immobilized on a solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab200208 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/1000 - 1/5000.
ELISA 1/2000 - 1/10000.

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References

This product has been referenced in:
  • Kim B  et al. TUT7 controls the fate of precursor microRNAs by using three different uridylation mechanisms. EMBO J 34:1801-15 (2015). Read more (PubMed: 25979828) »
  • Spång HC  et al. Heterodimeric Barnase-Barstar Vaccine Molecules: Influence of One versus Two Targeting Units Specific for Antigen Presenting Cells. PLoS One 7:e45393 (2012). WB . Read more (PubMed: 23028981) »

See all 4 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (recombinant human fibronectin domain)
Loading amount
0.1 µg
Specification
recombinant human fibronectin domain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Sep 04 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (yeast expressing a his tagged scFv)
Specification
yeast expressing a his tagged scFv
Preparation
Cell harvesting/tissue preparation method: yeast washed once in PBS
Sample buffer: PBS
Fixation
none
Permeabilization
No
Gating Strategy
sample was gated to include all yeast cells
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Abcam user community

Verified customer

Submitted Jun 24 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (Samples pre and post enzymatic his tag cleavage)
Loading amount
4 µg
Specification
Samples pre and post enzymatic his tag cleavage
Treatment
His tag purified supernatant followed by a reaction to cleave the his tag
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jun 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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