Overview

  • Product name
    Anti-6X His tag® antibody (FITC)
    See all 6X His tag® primary antibodies
  • Description
    Rabbit polyclonal to 6X His tag® (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: ELISA, WB, Flow Cytmore details
  • Immunogen

    Synthetic peptide: HHHHHH conjugated to KLH.

  • General notes

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Molar F/P ratio is 3.7:1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. PubMed: 21183632
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody (FITC) images

  • ab1206 staining 6X His tag® in Human endometrial carcinoma cells by Flow Cytometry. Cells were prepared by short trypsinisation in PBS/1% FBS/0.2% sodium azide and fixed with formaldehyde. The gate included all single cells. The sample was incubated with  a 6X His tag® primary antibody for 1 hour at 4°C.  Ab1206 was used as the secondary. On the image, the red line represents cells stained with ab1206 alone, and the blue line is the tested sample carrying a 6XHis tag®.  The small peak to the right represents cells that bound the tested sample.

    See Abreview

References for Anti-6X His tag® antibody (FITC) (ab1206)

This product has been referenced in:
  • Daringer NM  et al. Modular extracellular sensor architecture for engineering mammalian cell-based devices. ACS Synth Biol 3:892-902 (2014). ICC/IF . Read more (PubMed: 24611683) »
  • Yang L  et al. Prolidase directly binds and activates epidermal growth factor receptor and stimulates downstream signaling. J Biol Chem 288:2365-75 (2013). Read more (PubMed: 23212918) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Spodoptera frugiperda (SF9 cells) Cell (Baculovirus infected-cells. Baculovirus having a f)
Permeabilization
Yes - Saponin
Gating Strategy
Single cells (FS-A vs FS-H) then SS-A vs FS-A
Specification
Baculovirus infected-cells. Baculovirus having a f
Preparation
Cell harvesting/tissue preparation method: Cell culture gently pipetting up and down
Sample buffer: Pelleted in their own culture medium
Fixation
Formaldehyde
Username

Dr. Waldo Medina

Verified customer

Submitted May 02 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Detection of a HIS tagged protein bound to cells)
Specification
Detection of a HIS tagged protein bound to cells
Preparation
Cell harvesting/tissue preparation method: Trypsinisation and pre-incubation with HIS-tagged protein
Sample buffer: Serum free DMEM
Fixation
Nonne
Permeabilization
No
Gating Strategy
Live cell population
Username

Abcam user community

Verified customer

Submitted Jul 02 2012

I've assembled a list of the items that you would need forDengue Virus NS1 glycoprotein detection. Capture antibody ab41646,Anti-Dengue Virus NS1 glycoprotein antibody [DN3] Mouse monoclonal: http://www.abcam.com/Dengue-Virus-NS1-glycoprotein-antib...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (A431)
Specification
A431
Preparation
Cell harvesting/tissue preparation method: trypsin/EDTA
Sample buffer: PBS + 1 g/L BSA
Fixation
none
Permeabilization
No
Gating Strategy
no gating
Username

Dr. Benjamin Hackel

Verified customer

Submitted Sep 14 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (endometrial carcinoma)
Specification
endometrial carcinoma
Preparation
Cell harvesting/tissue preparation method: short trypsinisation
Sample buffer: PBS+1%FBS+0.02%NaN3
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
gate included all single cells
Username

Abcam user community

Verified customer

Submitted Oct 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Pichia pastoris Recombinant protein (yeast cell)
Specification
yeast cell
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Type
Indirect
Username

Abcam user community

Verified customer

Submitted May 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (yeast cells expressing a his tag on surface)
Specification
yeast cells expressing a his tag on surface
Permeabilization
No
Gating Strategy
gate included all yeast, both expressing and non-expressing
Username

Abcam user community

Verified customer

Submitted Jun 04 2008

The molecular weight of Rabbit IgG is ~150,000.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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