Anti-6X His tag® antibody (FITC) (ab1270)

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPBS with 0.2% BSA, 0.1% sodium azide
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/200 - 1/2000.

Target

  • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References for Anti-6X His tag® antibody (FITC) (ab1270)

ab1270 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email and I'm sorry to hear that you are experiencing difficulty with ab1270. Can you please tell me the lot number that you received (it is indicated on the vial)? Did you order directly from Abcam or through one of our distributors...

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Thank you for your enquiry. I regret to inform you that ab1270 has not yet been tested in IHC, only in ICC. There was an error on the datasheet which I have since updated. I apologize for this error and if you have any more questions, please contact us...

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The antibody is designed to react with 6 His at the N-terminus and C-terminus.

The method of fixation is unlikely to have significant effect on the His-tag. However, the fixation may influence the conformation of the tagged protein, and thereby, affect the accessibility of the His-tag. Thus, one must empirically determine the i...

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Cross-reactivities have not yet been reported, but this antibody should be specific for the 6x-His epitope tag.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"