Anti-6X His tag® antibody (FITC) (ab3554)

Overview

  • Product name
    Anti-6X His tag® antibody (FITC)
    See all 6X His tag® primary antibodies
  • Description
    Chicken polyclonal to 6X His tag® (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: WB, ELISA, IHC-Frmore details
  • Immunogen

    Synthetic peptide: HHHHHH conjugated to KLH.

  • General notes

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Molar F/P ratio is 3.6:1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    PBS, 0.2% BSA, 0.1% sodium azide, pH7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Myeloma
    unknown
  • Isotype
    IgY
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

Prior to conjugation, the antibodies were analyzed by Western Blot.

ELISA Use at an assay dependent concentration.

Prior to conjugation, the antibodies were analyzed by ELISA.

IHC-Fr 1/100.

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody (FITC) images

  • ab3554 staining 6X His tag® in rat spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with TritonX and blocked with 6% serum for 2 hours at 21°C. Samples were incubated with primary antibody (1/100 in 0.1M PBS + 0.3% Triton + 6% NGS) for 17 hours at 4°C.

    See Abreview

References for Anti-6X His tag® antibody (FITC) (ab3554)

ab3554 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 21°C
Sample
Rat Tissue sections (spinal cord tissue)
Specification
spinal cord tissue
Permeabilization
Yes - TritonX
Fixative
Paraformaldehyde
Username

Dr. Hayk Petrosyan

Verified customer

Submitted Jun 23 2014

I cannot advise performing western blotting with a FITC antibody although it is likely to work given a suitable secondary antibody that recognizes the primary chicken antiserum. The reason behind the fact that western blotting is a listed application i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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