For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
HHHHHHconjugated to KLH.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
Molar F/P ratio is 3.6:1.
Our Abpromise guarantee covers the use of ab3554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.
Prior to conjugation, the antibodies were analyzed by Western Blot.
|ELISA||Use at an assay dependent concentration.
Prior to conjugation, the antibodies were analyzed by ELISA.
ab3554 staining 6X His tag® in rat spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with TritonX and blocked with 6% serum for 2 hours at 21°C. Samples were incubated with primary antibody (1/100 in 0.1M PBS + 0.3% Triton + 6% NGS) for 17 hours at 4°C.
ab3554 has not yet been referenced specifically in any publications.