Anti-6X His tag® antibody [HIS-1] (ab49936)

Overview

  • Product name
    Anti-6X His tag® antibody [HIS-1]
    See all 6X His tag® primary antibodies
  • Description
    Mouse monoclonal [HIS-1] to 6X His tag®
  • Tested applications
    Suitable for: Flow Cyt, WB, Dot blot, ELISA, ICC/IFmore details
  • Immunogen

    Recombinant full length protein corresponding to 6X His tag®.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR224242. Lot numbers higher than GR224242 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Proprietary Purification
  • Purification notes
    Purified from Tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    HIS-1
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/3000.
Dot blot Use at an assay dependent concentration.

Use at an assay dependent concentration.

ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References for Anti-6X His tag® antibody [HIS-1] (ab49936)

ab49936 has not yet been referenced specifically in any publications.

Product Wall

Using 50ul of diluted antibody per well is an adequate amount although this amount may be optimized in your experiments. As this product is an unpurified ascites fluid, I would recommend using a slightly higher concentration of this product closer to 1...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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