Anti-6X His tag® antibody [HIS-1] (ab49936)

Overview

  • Product nameAnti-6X His tag® antibody [HIS-1]
    See all 6X His tag® primary antibodies
  • Description
    Mouse monoclonal [HIS-1] to 6X His tag®
  • Tested applicationsSuitable for: Flow Cyt, WB, Dot blot, ELISA, ICC/IFmore details
  • Immunogen

    Recombinant His-tagged fusion protein

  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR224242. Lot numbers higher than GR224242 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 15mM Sodium Azide
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberHIS-1
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/3000.
Dot blot Use at an assay dependent concentration.

Use at an assay dependent concentration.

ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References for Anti-6X His tag® antibody [HIS-1] (ab49936)

ab49936 has not yet been referenced specifically in any publications.

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Using 50ul of diluted antibody per well is an adequate amount although this amount may be optimized in your experiments. As this product is an unpurified ascites fluid, I would recommend using a slightly higher...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"