Anti-6X His tag® antibody [HIS-1] (ab49936)

Overview

  • Product name
    Anti-6X His tag® antibody [HIS-1]
    See all 6X His tag® primary antibodies
  • Description
    Mouse monoclonal [HIS-1] to 6X His tag®
  • Tested applications
    Suitable for: Flow Cyt, WB, Dot blot, ELISA, ICC/IFmore details
  • Immunogen

    Recombinant full length protein corresponding to 6X His tag®.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR224242. Lot numbers higher than GR224242 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Proprietary Purification
  • Purification notes
    Purified from Tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    HIS-1
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/3000.
Dot blot Use at an assay dependent concentration.

Use at an assay dependent concentration.

ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References

ab49936 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Using 50ul of diluted antibody per well is an adequate amount although this amount may be optimized in your experiments. As this product is an unpurified ascites fluid, I would recommend using a slightly higher concentration of this product closer to 1...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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