Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, pH 7
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/20000.
WB 1/1000 - 1/5000.

Target

  • RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody (HRP) images

  • All lanes : Anti-6X His tag® antibody (HRP) (ab1187) at 1/4000 dilution

    Lane 1 : 4ug Human His-tagged protein purified from E.coli
    Lane 2 : 2ug Human His-tagged protein purified from E.coli
    Lane 3 : 1ug Human His-tagged protein purified from E.coli
    Lane 4 : 0.5ug Human His-tagged protein purified from E.coli


    Observed band size : 136 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Mr Ohrt Thomas

    See Abreview

References for Anti-6X His tag® antibody (HRP) (ab1187)

This product has been referenced in:
  • Lee MJ  et al. Overlapping and distinct roles of Aspergillus fumigatus UDP-glucose 4-epimerases in galactose metabolism and the synthesis of galactose-containing cell wall polysaccharides. J Biol Chem 289:1243-56 (2014). WB . Read more (PubMed: 24257745) »
  • Schulte T  et al. The basic keratin 10-binding domain of the virulence-associated pneumococcal serine-rich protein PsrP adopts a novel MSCRAMM fold. Open Biol 4:130090 (2014). ELISA . Read more (PubMed: 24430336) »

See all 29 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse pancreatic cancer primary cell lines)
Loading amount 25 µg
Specification mouse pancreatic cancer primary cell lines
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 09 2011

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 0.1 µg
Gel Running Conditions Reduced Denaturing
Sample Human Recombinant protein (purification sur colonne HIS Trap)
Specification purification sur colonne HIS Trap
Blocking step caséine as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 30 2013

Dank für Ihre Antwort.

Ich habe zwei weitere anti-HIS tag Antikörper, die für ELISA getestet und garantiert sind und auch HRP gekoppelt sind.

ab1269

http://www.abcam.com/index.html?datasheet=1269 (or use the...

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Vielen Dank für das Durchführen der Kontrollen.

Ich stimme Ihnen zu dass eine 1:40000 Verdünnung hoch genug sein sollte und nicht zu einem hohen Hintergrund führen darf.

Ich weiß nicht, was mit dem Röh...

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We have a number of antibodies in our catalog that might be of interest to you. I would like to direct you to the search facility on our website (www.abcam.com).

Please enter the name of the protein into the search box at the top of the home...

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Thank you for contacting us.
I am sorry to hear you are having problems with one of our products. I appreciate the details provided. I am afraid that product ab41616 is not guaranteed to work in ELISA. We have tested this antibody in WB and ICC, b...

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Thank you for contacting Abcam regarding ab1187.


I am sorry that you have been experiencing difficulties with this antibody in your ELISA experiments. Although the results resemble a somewhat linear response, I am not convinced the anti...

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Thank you for contacting us.

As this antibody is delivered in an amber vial, the answer is yes. However, this does not mean that you cannot use this product as usual.

I hope this information is helpful to you. Please do not hesita...

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Thank you for contacting us. I've assembled a list of the items that you would need forDengue Virus NS1 glycoprotein detection. Capture antibody ab41646,Anti-Dengue Virus NS1 glycoprotein antibody [DN3] Mouse monoclonal: http://www.abcam.com/Dengu...

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Thank you for calling Abcam earlier. After having spoken to the lab, I would advise you not to store ab1187 at at -20C as it will cause the product to lose some functionality. If there is anything else I can help you with, please let me know.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"