Overview

  • Product name
    Anti-6X His tag® antibody (HRP)
    See all 6X His tag® primary antibodies
  • Description
    Rabbit polyclonal to 6X His tag® (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Immunogen

    Synthetic peptide: HHHHHH conjugated to KLH.

  • General notes
    HIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, pH 7
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/20000.
WB 1/1000 - 1/5000.

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody (HRP) images

  • All lanes : Anti-6X His tag® antibody (HRP) (ab1187) at 1/4000 dilution

    Lane 1 : 4ug Human His-tagged protein purified from E.coli
    Lane 2 : 2ug Human His-tagged protein purified from E.coli
    Lane 3 : 1ug Human His-tagged protein purified from E.coli
    Lane 4 : 0.5ug Human His-tagged protein purified from E.coli


    Observed band size : 136 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Mr Ohrt Thomas

    See Abreview

References for Anti-6X His tag® antibody (HRP) (ab1187)

This product has been referenced in:
  • Schulte T  et al. The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation. Sci Rep 6:32371 (2016). WB . Read more (PubMed: 27582320) »
  • Hannan JP  et al. Mapping the Complement Factor H-Related Protein 1 (CFHR1):C3b/C3d Interactions. PLoS One 11:e0166200 (2016). Read more (PubMed: 27814381) »

See all 31 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse pancreatic cancer primary cell lines)
Loading amount
25 µg
Specification
mouse pancreatic cancer primary cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 09 2011

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
0.1 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Recombinant protein (purification sur colonne HIS Trap)
Specification
purification sur colonne HIS Trap
Blocking step
caséine as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 30 2013

Thank you for calling Abcam earlier. After having spoken to the lab, I would advise you not to store ab1187 at at -20C as it will cause the product to lose some functionality due to the HRP conjugation.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Purified protein (purified His-tagged lipase, SPINK1)
Loading amount
1 µg
Specification
purified His-tagged lipase, SPINK1
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Miklos Sahin-Toth

Verified customer

Submitted Aug 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuronal cell)
Loading amount
20 µg
Specification
neuronal cell
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Feb 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Purified protein (Purified His-tagged extracellular human LDLR)
Loading amount
0.1 µg
Specification
Purified His-tagged extracellular human LDLR
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 25 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Escherichia coli Purified protein (BL21(DE3))
Specification
BL21(DE3)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Type
Sandwich (Capture)
Username

Abcam user community

Verified customer

Submitted Jan 27 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (BL21(DE3))
Loading amount
0.01 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 30 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Purified protein (recombinant human fibronectin domain expressed in)
Loading amount
1 µg
Specification
recombinant human fibronectin domain expressed in
Gel Running Conditions
Reduced Denaturing (12% BisTris NuPAGE gel)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Sep 09 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Rat Serum (injected his-yeagged peptide)
Specification
injected his-yeagged peptide
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Type
Sandwich (Detection)
Username

Dr. Sandra Reichstetter

Verified customer

Submitted Dec 11 2007

1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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