HRP Anti-6X His tag® antibody (ab1269)
Key features and details
- HRP Goat polyclonal to 6X His tag®
- Suitable for: WB, ELISA, ICC
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
HRP Anti-6X His tag® antibody
See all 6X His tag® primary antibodies -
Description
HRP Goat polyclonal to 6X His tag® -
Host species
Goat -
Conjugation
HRP -
Tested applications
Suitable for: WB, ELISA, ICCmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
-
General notes
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
1/1000 - 1/30000.
|
ELISA | (1) |
1/10000 - 1/100000.
|
ICC |
1/200 - 1/500.
|
Notes |
---|
WB
1/1000 - 1/30000. |
ELISA
1/10000 - 1/100000. |
ICC
1/200 - 1/500. |
Target
-
Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (8)
ab1269 has been referenced in 8 publications.
- Shen M et al. Structural basis for the multi-activity factor Rad5 in replication stress tolerance. Nat Commun 12:321 (2021). PubMed: 33436623
- Xu X et al. Structural insights into sodium transport by the oxaloacetate decarboxylase sodium pump. Elife 9:N/A (2020). PubMed: 32459174
- Lee CH et al. IgG Fc domains that bind C1q but not effector Fc? receptors delineate the importance of complement-mediated effector functions. Nat Immunol 18:889-898 (2017). PubMed: 28604720
- Li Y & Smolke CD Engineering biosynthesis of the anticancer alkaloid noscapine in yeast. Nat Commun 7:12137 (2016). WB . PubMed: 27378283
- Seijsing J et al. Robust expression of the human neonatal Fc receptor in a truncated soluble form and as a full-length membrane-bound protein in fusion with eGFP. PLoS One 8:e81350 (2013). ELISA . PubMed: 24260574
- de Vrij J et al. A cathepsin-cleavage site between the adenovirus capsid protein IX and a tumor-targeting ligand improves targeted transduction. Gene Ther 19:899-906 (2012). PubMed: 22011643
- Jevnikar Z et al. Cathepsin X cleavage of the {beta}2 integrin regulates talin-binding and LFA-1 affinity in T cells. J Leukoc Biol 90:99-109 (2011). PubMed: 21454358
- Uil TG et al. A lentiviral vector-based adenovirus fiber-pseudotyping approach for expedited functional assessment of candidate retargeted fibers. J Gene Med 11:990-1004 (2009). ELISA . PubMed: 19757488