Overview

  • Product name
    Anti-6X His tag® antibody (HRP)
    See all 6X His tag® primary antibodies
  • Description
    Chicken polyclonal to 6X His tag® (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Immunogen

    Synthetic peptide: HHHHHH conjugated to KLH.

  • General notes
    HIS-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    50 mM HEPES pH 7.0, 0.1 M NaCl, 0.2% BSA, 0.01% Thimerosal
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Myeloma
    unknown
  • Isotype
    IgY
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. 1/1000.
ELISA Use at an assay dependent concentration. a 1/10000 dilution gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti-peptide purification.

Target

  • Relevance
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Cellular localization
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • Alternative names
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

References

This product has been referenced in:
  • Allan CM  et al. A conserved START domain coenzyme Q-binding polypeptide is required for efficient Q biosynthesis, respiratory electron transport, and antioxidant function in Saccharomyces cerevisiae. Biochim Biophys Acta 1831:776-791 (2013). Read more (PubMed: 23270816) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (protein produced in mammalian expression system)
Loading amount
1 µg
Specification
protein produced in mammalian expression system
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Nov 29 2007

Thank you for your enquiry. Yes, this product will detect N-terminal HIS tags.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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