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Western blot - 68kDa Neurofilament antibody - Neuronal Marker (ab9035)
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Immunocytochemistry/ Immunofluorescence - 68kDa Neurofilament antibody - Neuronal Marker (ab9035)
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Product Name
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68kDa Neurofilament antibody - Neuronal Marker
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See all 68kDa Neurofilament antibodies (13)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to 68kDa Neurofilament - Neuronal Marker
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Immunogen
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Pig intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and this cytoskeletal material was dissolved in 6M urea. NF-L was purified by ion exchange chromatography, followed by preparative gel electrophoresis.
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Reacts with
(species key)
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Mamm, Bird
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Specificity
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Specifically recognizes the light neurofilament subunit (~68 kDa).
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Tested applications
(see key)
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ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/500.
IHC-F: 1/1000.
IHC-P: 1/1000.
IHC-Fr: 1/1000.
WB: 1/5000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Developmental Biology >> Lineage specification >> Ectoderm
Neuroscience >> Cell Type Marker >> Neuron marker >> Axon marker
Stem Cells >> Lineage Markers >> Ectoderm
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class IV >> Neurofilaments
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Intermediate Filaments
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
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Relevance
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Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF L, NF M and NF H. NF L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Neurofilaments constitute the main structural elements of neuronal axons and dendrites. Neurofilaments are composed of three major subunits referred to as the neurofilament triplet, with molecular weights of 68kD, 160kD and 200kD. Neurofilament subunits are present in neurons, neuronal processes, peripheral nerves and sympathetic ganglion cells.
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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n/a
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this 68kDa Neurofilament antibody - Neuronal Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for 68kDa Neurofilament antibody - Neuronal Marker images, references, products related to ab9035 and other tools.
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68kDa Neurofilament antibody - Neuronal Marker images:
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 Western blot - 68kDa Neurofilament antibody - Neuronal Marker (ab9035)
68kDa Neurofilament antibody - Neuronal Marker (ab9035) at 1/20000 dilution + whole rat cerebellum homogenate
A prominent band at ~68kDa corresponds to rodent NF-L. Human, cow and bovine NF-L run a little slower, at about 70kDa.
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Immunocytochemistry/ Immunofluorescence - 68kDa Neurofilament antibody - Neuronal Marker (ab9035)
ab9035 at a 1/300 dilution staining rat hippocampal organotypic slice cultures by ICC/IF. The primary antibody was incubated with the cells for 120 hours (this time allows for the antibody to penetrate a layer of glial cells that grows while the slice is in culture). Bound antibody is detected using a Cy3 conjugated Indocarbocyanine. This image is courtesy of an Abreview submitted by Dr Luis Craveiro
See Abreview
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References for 68kDa Neurofilament antibody - Neuronal Marker (ab9035)
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This product has been used in: (two most recent references)
Clements KM et al. Cellular and histopathological changes in the infrapatellar fat pad in the monoiodoacetate model of osteoarthritis pain. Osteoarthritis Cartilage 17:805-12 (2009). PubMed: 19114312
Harris J et al. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62 (1991). PubMed: 1724473
See all 3 publication references for this product.
If you publish research using ab9035 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to 68kDa Neurofilament
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