FITC Anti-DNA/RNA Damage antibody [15A3] (ab183393)
Key features and details
- FITC Mouse monoclonal [15A3] to DNA/RNA Damage
- Suitable for: ICC/IF, IHC-P, IHC-Fr
- Reacts with: Species independent
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
FITC Anti-DNA/RNA Damage antibody [15A3]
See all DNA/RNA Damage primary antibodies -
Description
FITC Mouse monoclonal [15A3] to DNA/RNA Damage -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
15A3 binds with high specificity and affinity to oxo8dG (8-hydroxy-2'-deoxyguanosine), oxo8Gua (8-oxo-7,8-dihydroguanine), and oxo8G (8-oxo-7,8-dihydroguanosine) present in biological fluids with Kaff values of 9.2 x 108 M-1, 4.7 x' 17 M-1, and 2.1 x 108 M-1, respectively. -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to DNA/RNA Damage. 8-hydroxy-guanosine-BSA and -casein conjugates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
15A3 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 1 - 10 µg/ml.
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IHC-Fr |
Use a concentration of 1 - 10 µg/ml.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 1 - 10 µg/ml. |
IHC-Fr
Use a concentration of 1 - 10 µg/ml. |
Target
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Relevance
In intact animals, lesions (adducts) excised from DNA are transported from the cell through the circulation and excreted in urine. In bacteria, DNA adducts are excreted directly into the medium. In either case, the adducts can be assayed as a measure of oxidative damage to DNA. In particular, Oxo-8-dG (8-Oxo-7,8-dihydro-2'-deoxyguanosine) serves as an excellent marker for DNA damage produced by oxidants because it represents one of the major products generated by a wide array of treatments associated with oxidant damage such as that produced by irradiation and various carcinogens and because it is implicated in spontaneous transversion mutagenesis. Oxo-8-Gua (8-oxo-7,8-dihydroguanine) is one of the most common DNA lesions resulting from reactive oxygen species and can result in a mismatched pairing with adenine resulting in G to T and C to A substitutions in the genome. In humans, it is primarily repaired by DNA glycosylase OGG1. It can be caused by ionizing radiation, in connection with oxidative metabolism. Oxo-8-G (8-oxo-7,8-dihydroguanosine) is classified as an oxidized ribonucleotide, and is primarily used in studies of oxidative RNA damage and associated RNA repair and RNA turnover mechanisms within the cell. In the cell, Oxo-8-G RNA lesions are formed by reaction with reactive oxygen species (ROS) generated either via normal oxidative metabolic processes, UV ionizing radiation, or exposure to oxidative agents. Oxidative RNA damage can lead to defects in protein synthesis, for example, decreased rates of protein synthesis and production of aggregated or truncated peptides, with important implications in aging and neurodegenerative disorders and artherosclerosis. -
Alternative names
- 7,8-Dihydro-8-oxo-2'-deoxyguanosine antibody
- 7,8-Dihydro-8-oxodeoxyguanosine antibody
- 7,8-Dihydro-8-oxoguanine antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed mouse inflamed colon labelling DNA damage with ab183393 at 1/1000000 dilution, followed by Biotin Goat Anti-Mouse at 1/2000 dilution. Counterstained with Mayer Hematoxylin (purple/blue) nuclear stain.
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Immunohistochemical analysis of paraffin-embedded mouse blackskin tissue labelling DNA damage with ab183393 at 1/100 dilution, followed by FITC Goat Anti-Mouse (green) at 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab183393 has been referenced in 6 publications.
- Quesada I et al. Antioxidant and Anti-atherogenic Properties of Prosopis strombulifera and Tessaria absinthioides Aqueous Extracts: Modulation of NADPH Oxidase-Derived Reactive Oxygen Species. Front Physiol 12:662833 (2021). PubMed: 34335290
- Fan ZQ et al. Oxidative Stress Induced Osteocyte Apoptosis in Steroid-Induced Femoral Head Necrosis. Orthop Surg 13:2145-2152 (2021). PubMed: 34559465
- Rodius S et al. Fisetin protects against cardiac cell death through reduction of ROS production and caspases activity. Sci Rep 10:2896 (2020). PubMed: 32076073
- Kurita H et al. Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation. PLoS One 15:e0232724 (2020). PubMed: 32374749
- Tempka D et al. Downregulation of PARP1 transcription by CDK4/6 inhibitors sensitizes human lung cancer cells to anticancer drug-induced death by impairing OGG1-dependent base excision repair. Redox Biol 15:316-326 (2018). PubMed: 29306194
- Park EM et al. Assay of excised oxidative DNA lesions: isolation of 8-oxoguanine and its nucleoside derivatives from biological fluids with a monoclonal antibody column. Proc Natl Acad Sci U S A 89:3375-9 (1992). PubMed: 1565629