Anti-8 Hydroxyguanosine antibody (ab10802)

Hola buenos días.
Acabo de procesar un pedido con referencia ab10802 que es un anti-8 hydroxyguanosine antibody, y me gustaría saber si este anticuerpo policlonal de goat funciona con un anticuerpo secundario alexa anti-goat, ya que veo que la foto que tienen en su web es de tejido y creo que es con DAB, y me gustaría hacer la inmuno con fluorescencia.
Si pueden ayudarme, muchas gracias, un saludo

ANSWER:

Gracias por contactarnos.

Siempre que el anticuerpo primario y el secundario sean compatibles (mismo isotipo y secundario dirigido contra la especie del primario, ambos garantizados en la misma aplicación), el primario será detectado por un secundario independientemente de que éste esté conjugado a una enzima o a un fluorocromo.

En cualquier caso, si el anticuerpo no funcionara correctamente, éste tiene una garantía de 6 meses por la que os reemplazaríamos o reembolsaríamos el importe del mismo en caso de no comportarse como se especifica en su datasheet.

Espero que esta información sea de utilidad. En caso contrario, no dudes en contactarnos de nuevo.

I am writing to see if you have heard back about the cross reactivity of the following antibodies:ab48508, ab93701. Please see the email below for a refresher regarding our original exchange which took place a month ago. Thank you for your time.

ANSWER:

Thank you for your reply.
I have still not heard back from all of those products. I am going to send email reminders and follw up with what is happening. I will get back to you as soon as I find out anything.
Thank you for your patience.

Phoned to ask if product had been tested in analogues. Also wondering if Anti-8 Hydroxyguanosine antibody would recognize 8-Hydroxy-2'-deoxyguanosine antibody.

ANSWER:

Thank you for contacting us.

I have been in contact with the labs about the products that you had called about. Not all of my requests have been returned yet, however I have received some information and wanted to pass that on to you.

ab26842: This has not been tested against different analogues of 8-hydroxy-2' deoxyguanosine for cross reactivity
ab10802: This antibody will recognize 8-hydroxy-2'-deoxyguanosine as well as 8 Hydroxyguanosine.
ab62623: This antibody will recognize 8-Hydroxy-2'-deoxyguanosine as well as 8 Hydroxyguanosine.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Hello Sir,  I am the technical support of seajet , I am writing to inquirying a antibody,the Cat No is ab10802. The customer wants to detect a  protein in Mouse brain tissue, but the data did not specify the reactivity species.So can this antibody  be  used to detect protein in Mouse(WB/IHC)?    Looking forward to your reply        kindly regards  

ANSWER:

Thank you for your enquiry and your interest in our products.

I can confirm that this antibody recognizes 8 Hydroxyguanosine which is a modified nucleotide and not a protein. Therefore, it is predicted to react with a wide range of different species (including mouse). As the datasheet indicates, ab10802 has been characterized IHC-P, ELISA, ICC/IF. However, it has not been tested in Western blot application yet.

If you need any further assistance in the future, please do not hesitate to contact me.  

BATCH NUMBER 93912 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Non-specific staining. Negative controls (with primary omitted) have very low staining (i.e. very little background staining). Staining is strongest (and also non-specific) around edge of section. SAMPLE Rat kidneys with and without necrosis and inflammation PRIMARY ANTIBODY Goat polyclonal to 8 hyroxyguanosine from Abcam (see above) DETECTION METHOD ABC-HRP from Dako, and DAB from Dako POSITIVE AND NEGATIVE CONTROLS USED Hoping to use the kidney sections as positive (necrosis/inflammation) and negative (healthy) controls for other experiments. ANTIBODY STORAGE CONDITIONS 4C fridge FIXATION OF SAMPLE 48 Hours formalin ANTIGEN RETRIEVAL Pressurised microwave at 110C for 2mins (once temp. reached) in pH6 citrate buffer. Also tried without retrieval - staining is lesser, but still non-specific BLOCKING CONDITIONS 30 mins normal rabbit serum (1:20 dilution) SECONDARY ANTIBODY Biotinylated rabbit anti-goat from dako. 30 mins. PBS with 0.05% tween wash between all steps. HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 6 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Dilution range; 1/200 1/300 1/400 1/500 Non-charged slides - however all sections float off Same staining also seen in pancreas section ADDITIONAL NOTES Slides used are Thermo-Electron Shandon Microscpe Slides Colorfrost Plus (charged)

ANSWER:

I have received very useful information from the laboratory and agree with their diagnosis of the problem. We suspect the problem is due to the endogenous biotin produced in kidney tissue; upon usage of ABC and HRP, the ABC amplification component avidin binds to the endogenous biotin protein and hence causes non specific binding and staining.

We therefore recommend you try a secondary antibody which is HRP conjugated to directly bind the primary antibody.

I hope this information will help you, please do not hesitate to contact me if you require further help,