![Competitive ELISA - 8-hydroxy-2' deoxyguanosine antibody [7D7E4] (ab26842) image](#ab26842_ELISA_1.jpg)
![Immunohistochemistry - 8-hydroxy Guanine antibody [7D7E4] (ab26842) image](#ab26842_IHC_1.jpg)
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Product name
Anti-8-hydroxy-2' deoxyguanosine antibody [7D7E4]
Description
Mouse monoclonal [7D7E4] to 8-hydroxy-2' deoxyguanosine
Specificity
ab26842 recognizes the oxidized epitope (8-OH dG) and not the 2'-deoxyguanosine (dG)
Tested applications
ELISA, IHC-P, ICC/IFmore details
Immunogen
8-hydroxy-2'deoxyguanisine conjugated to carrier protein.
Positive control
Hamster oral epithelial cancerous tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
7D7E4
Isotype
IgG1
Research areas
Metabolism >> Pathways and Processes >> Redox metabolism >> Oxidative stress
Cell Biology >> Other Antibodies >> Oxidative Stress
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides
Applications
Show applications key
Our Abpromise guarantee covers the use of ab26842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/5000. For detecting 0.1ng of 8-OHdG-BSA.
IHC-P
IHC-P: 1/50.IHC-P: 1/50.
ICC/IF: Use at an assay dependent dilution.
Target
Relevance
Oxidative damage of DNA or RNA by reactive oxygen and nitrogen species produces 8-hydroxy Guanine. This can serve as a measure of oxidative stress.
Anti-8-hydroxy-2' deoxyguanosine antibody [7D7E4] images:
Competitive ELISA - 8-hydroxy-2' deoxyguanosine antibody [7D7E4] (ab26842)
![Competitive ELISA - 8-hydroxy-2' deoxyguanosine antibody [7D7E4] (ab26842)](/ps/datasheet/Images/26/ab26842/ab26842_ELISA_1.jpg)
Testing ab26842 8-OHdG mouse monoclonal antibody with competitive ELISA using dA, dT, dC and dG as competitive antigen
Immunohistochemistry - 8-hydroxy Guanine antibody [7D7E4] (ab26842)
![Immunohistochemistry - 8-hydroxy Guanine antibody [7D7E4] (ab26842)](/ps/datasheet/Images/26/ab26842/ab26842_IHC_1.jpg)
1:50 dilution
ab26842, staining 8-hydroxy Guanine in hamster oral epithelial cancerous tissue, by Immunohistochemistry. The image shows that that ab26842 can stain DNA damage in the cytoplasm and the nucleus
References for Anti-8-hydroxy-2' deoxyguanosine antibody [7D7E4] (ab26842)
This product has been referenced in:
- de Sablet T et al. Phylogeographic origin of Helicobacter pylori is a determinant of gastric cancer risk. Gut 60:1189-95 (2011). IHC-P.Read more (PubMed: 21357593) »
- Moiseeva O et al. Mitochondrial dysfunction contributes to oncogene-induced senescence. Mol Cell Biol 29:4495-507 (2009). ICC/IF.Read more (PubMed: 19528227) »
See all 2 publications for this product
Publishing research using ab26842? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"