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Anti-8-hydroxy-2' deoxyguanosine antibody [7D7E4] (ab26842)

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Overview

Product name

Anti-8-hydroxy-2' deoxyguanosine antibody [7D7E4]

Description

Mouse monoclonal [7D7E4] to 8-hydroxy-2' deoxyguanosine

Specificity

ab26842 recognizes the oxidized epitope (8-OH dG) and not the 2'-deoxyguanosine (dG)

Tested applications

ELISA, IHC-P, ICC/IFmore details

Immunogen

8-hydroxy-2'deoxyguanisine conjugated to carrier protein.

Positive control

Hamster oral epithelial cancerous tissue.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Monoclonal

Clone number

7D7E4

Isotype

IgG1

  • Competitive ELISA - 8-hydroxy-2' deoxyguanosine antibody [7D7E4] (ab26842)Competitive ELISA - 8-hydroxy-2' deoxyguanosine antibody [7D7E4] (ab26842) image (enlarge)

  • Immunohistochemistry - 8-hydroxy Guanine antibody [7D7E4] (ab26842)Immunohistochemistry - 8-hydroxy Guanine antibody [7D7E4] (ab26842) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab26842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    ELISA

     ELISA: 1/5000. For detec...Read more →

    ELISA: 1/5000. For detecting 0.1ng of 8-OHdG-BSA.

  • IHC-P

     IHC-P: 1/50.

    IHC-P: 1/50.

  • ShowHide

    ICC/IF

     ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent dilution.

Target

Relevance

Oxidative damage of DNA or RNA by reactive oxygen and nitrogen species produces 8-hydroxy Guanine. This can serve as a measure of oxidative stress.

Anti-8-hydroxy-2' deoxyguanosine antibody [7D7E4] images:

  Competitive ELISA - 8-hydroxy-2' deoxyguanosine antibody [7D7E4] (ab26842)

Competitive ELISA - 8-hydroxy-2' deoxyguanosine antibody [7D7E4] (ab26842)

Testing ab26842 8-OHdG mouse monoclonal antibody with competitive ELISA using dA, dT, dC and dG as competitive antigen

  Immunohistochemistry - 8-hydroxy Guanine antibody [7D7E4] (ab26842)

Immunohistochemistry - 8-hydroxy Guanine antibody [7D7E4] (ab26842)

1:50 dilution

ab26842, staining 8-hydroxy Guanine in hamster oral epithelial cancerous tissue, by Immunohistochemistry. The image shows that that ab26842 can stain DNA damage in the cytoplasm and the nucleus

References for Anti-8-hydroxy-2' deoxyguanosine antibody [7D7E4] (ab26842)

This product has been referenced in:

  • de Sablet T  et al. Phylogeographic origin of Helicobacter pylori is a determinant of gastric cancer risk. Gut 60:1189-95 (2011). IHC-P.Read more (PubMed: 21357593) »
  • Moiseeva O  et al. Mitochondrial dysfunction contributes to oncogene-induced senescence. Mol Cell Biol 29:4495-507 (2009). ICC/IF.Read more (PubMed: 19528227) »

See all 2 publications for this product

Publishing research using ab26842? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"