Anti-8 Hydroxyguanosine antibody [7D7E4] (ab26842)

Overview

• Product nameAnti-8 Hydroxyguanosine antibody [7D7E4]See all 8 Hydroxyguanosine primary antibodies ...
• Description
  Mouse monoclonal [7D7E4] to 8 Hydroxyguanosine
• Specificityab26842 recognizes the oxidized epitope (8-OH dG) and not the 2'-deoxyguanosine (dG)
• Tested applicationsELISA, IHC-P, ICC/IF more details
• Immunogen

  8-hydroxy-2'deoxyguanisine conjugated to carrier protein.

• Positive controlHamster oral epithelial cancerous tissue.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Monoclonal
• Clone number7D7E4
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA / Nucleotides

  • Cell Biology
  • Other Antibodies
  • Oxidative Stress

Applications

Target

• Relevance8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease. 8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress.

• Alternative names
  8 hydroxy 2’ deoxyguanosine antibody8 hydroxyguanine antibody8 hydroxyguanosine antibody

  8 OHG antibody8-OHG antibody8OG antibody8OHdG antibody8OHG antibody

  see all

Anti-8 Hydroxyguanosine antibody [7D7E4] images

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  Competitive ELISA - 8-hydroxy-2' deoxyguanosine antibody [7D7E4] (ab26842)Testing ab26842 8-OHdG mouse monoclonal antibody with competitive ELISA using dA, dT, dC and dG as competitive antigen
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  Immunohistochemistry - Anti-8 Hydroxyguanosine antibody [7D7E4] (ab26842)
  ab26842 at 1/50 dilution, staining 8 Hydroxyguanosine in hamster oral epithelial cancerous tissue, by Immunohistochemistry. The image shows that that ab26842 can stain DNA damage in the cytoplasm and the nucleus.
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8 Hydroxyguanosine antibody [7D7E4] (ab26842)Image from Barret M et al., Fibrogenesis Tissue Repair. 2012 May 28;5(1):8. doi: 10.1186/1755-1536-5-8. Fig 5.; 28 May 2012, Fibrogenesis & Tissue Repair 2012, 5:8
  Immunohistochemical analysis of pig esophageal tissue, staining 8 Hydroxyguanosine with ab26842. ab97240 - Goat polyclonal to Mouse IgG1 - heavy chain (HRP) was used as the secondary antibody.