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Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)

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Overview

Product name

Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1]

Description

Mouse monoclonal [N45.1] to 8-Hydroxy-2'-deoxyguanosine

Specificity

Nineteen analogues of 8-Hydroxy-2'-deoxyguanosine {guanosine (G), 7-methyl-G, 6-SH-G, 8-bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine (Gua), O6-methyl-Gua, 8-OHGua, uric acid, Urea, creatine, creatinine} demonstrate no cross-reactivity. Only 8-sulfhydryl-G and 8-OHG demonstrate minimal cross-reactivity (less than 1%).

Tested applications

ICC, IHC-Pmore details

Immunogen

Chemical / Small Molecule: 8-Hydroxy-2'-deoxyguanosine conjugated Keyhole Limpet Hemocyanin

Positive control

Epidermis from hairless mouse treated for 4 weeks with chronic UVB irradiation, or kidney treated with Fe-NTA.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 1% BSA, 10mM PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Ammonium Sulphate Precipitation

Clonality

Monoclonal

Clone number

N45.1

Isotype

IgG1

Light chain type

kappa

Research areas

Metabolism >> Pathways and Processes >> Redox metabolism >> Oxidative stress
Cell Biology >> Other Antibodies >> Oxidative Stress
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)Immunocytochemistry/ Immunofluorescence - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab48508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

8-hydroxy-2'-deoxyguanosine is a biomarker of oxidative DNA damage.

Alternative names

  • 8 OHdG antibody
  • 8-OHdG antibody
  • 8OHdG antibody

Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)

Ab48508, at a concentration of 5 µg/ml, staining 8-Hydroxy-2'-deoxyguanosine in epidermis from hairless mouse after 4 weeks of treatment by chronic UVB irradiation.

  Immunocytochemistry/ Immunofluorescence - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)

Immunocytochemistry/ Immunofluorescence - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)

ab48508 staining 8-Hydroxy-2'-deoxyguanosine in human spermatozoa by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in paraformaldehyde, permeabilized using triton in 4% BSA for 15 minutes at room temperature, blocked with 4% BSA for 30 minutes at 20°C and then incubated using ab48508 at a 1/200 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 350 conjugated goat anti-mouse monoclonal, used at a 1/250 dilution.

Staining from left to right: 1. TUNEL 2. ab48508 3. DIC 4. Merge.

Image courtesy of an anonymous Abreview.

See Abreview

References for Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)

This product has been referenced in:

  • DeNicola GM  et al. Oncogene-induced Nrf2 transcription promotes ROS detoxification and tumorigenesis. Nature 475:106-9 (2011). IHC-P.Read more (PubMed: 21734707) »
  • Sukhanov S  et al. Differential requirement for nitric oxide in IGF-1-induced anti-apoptotic, anti-oxidant and anti-atherosclerotic effects. FEBS Lett : (2011). IHC-P.Read more (PubMed: 21872589) »

See all 5 publications for this product

Publishing research using ab48508? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"