Anti-8 Hydroxyguanosine antibody [15A3] (ab62623)

Overview

  • Product nameAnti-8 Hydroxyguanosine antibody [15A3]
    See all 8 Hydroxyguanosine primary antibodies
  • Description
    Mouse monoclonal [15A3] to 8 Hydroxyguanosine
  • Tested applicationsSuitable for: IHC-P, ELISA, ICC/IF, IHC-Fr, IHC-FoFrmore details
  • Species reactivity
    N/A
  • Immunogen

    Other Immunogen Type corresponding to 8 Hydroxyguanosine. 8-hydroxy-guanosine-BSA and –casein conjugates

  • General notes

    Please see the protocol booklet link below for recommended IHC and ICC staining procedure

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number15A3
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 10 µg/ml.

Dilute antibody in PBS containing 0.3% Triton X-100, 0.08% sodium azide and 2% normal goat serum.

ELISA Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.

PubMed 25479606

 

IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21824519

Target

  • Relevance8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease. 8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress.
  • Alternative names
    • 8 hydroxy 2’ deoxyguanosine antibody
    • 8 hydroxyguanine antibody
    • 8 hydroxyguanosine antibody
    • 8 OHG antibody
    • 8-OHG antibody
    • 8OG antibody
    • 8OHdG antibody
    • 8OHG antibody
    see all

Anti-8 Hydroxyguanosine antibody [15A3] images

  • Immunohistochemical analysis of murine brain tissue 24 hours after recirculation following ischemia. 8 Hydroxyguanosine was stained using ab62623 at 1/1000 dilution. An AlexaFluor®488-conjugated anti-mouse IgG (1/500) was used as the secondary antibody.

  • Left panel: ab62623 staining 8-hydroxyguanosine in ischemic rat brain tissue
    Centre panel: DAPI staining
    Right panel: merged
  • Immunohistochemical analysis of PFA-fixed paraffin-embedded rat femural tissue, labelling 8-Hydroxyguanosine with ab62623 at a dilution of 1/50 incubated for 13 hours at 4°C in 1% BSA in TBS. Heat mediated antigen retrival was performed via Tris-EDTA pH 9.0. Blocking was via ab93695 ABC kit incubated at 1% for 20 minutes at room temperature. A sexondary was not used, but ab93695 detection kit was used for signal amplification.

    See Abreview

  • ab62623 staining 8 Hydroxyguanosine in rat liver tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with Proteinase K solution and then blocking for 20 minutes at 37°C was performed. The primary antibody was diluted 1/4000 and incubated with sample for 2 hours at 37°C. A Biotin conjugated rabbit polyclonal to mouse IgG was used as secondary antibody at 1/200 dilution.

    See Abreview

Protocols

References for Anti-8 Hydroxyguanosine antibody [15A3] (ab62623)

This product has been referenced in:
  • Lood C  et al. Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease. Nat Med N/A:N/A (2016). IF . Read more (PubMed: 26779811) »
  • Chung HK  et al. The indole derivative NecroX-7 improves nonalcoholic steatohepatitis in ob/ob mice through suppression of mitochondrial ROS/RNS and inflammation. Liver Int 35:1341-53 (2015). Read more (PubMed: 25443620) »

See all 13 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (hepatocytes)
Permeabilization Yes - 0.1% Triton X-100 in TBS for 5-10 minutes
Specification hepatocytes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative Paraformaldehyde
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Verified customer

Submitted Aug 09 2016

Application Immunocytochemistry
Sample Oncorhynchus mykiss Cell (Spermatozoa)
Permeabilization Yes - 0,3%Triton-X100 in PBS1X
Specification Spermatozoa
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 4°C
Fixative Paraformaldehyde
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Submitted Oct 06 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Bone tissue (Femur))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Specification Bone tissue (Femur)
Blocking step Ab93695 ABC kit as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Mr. Helder Fonseca

Verified customer

Submitted Sep 11 2015

Application ELISA
Sample Human Cell (immortalized proximal tubule HK2 cells)
Specification immortalized proximal tubule HK2 cells
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 100% · Temperature: 24°C
Type Other
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Submitted Jul 22 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample Mouse Cell (Hepatocyte)
Specification Hepatocyte
Permeabilization No
Fixative Paraformaldehyde
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Submitted Jan 23 2014

We added IHC of paraffin-embedded tissue to the applications section of the ab62623 datasheet based on reviews we received from customers. These are linked on the main online datasheet. Each customer used a different method for antigen retrieval. One u...

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (B16F10 melanoma)
Specification B16F10 melanoma
Fixative 4% Formalin
Permeabilization Yes - 0.1% SDS
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

Submitted Mar 15 2013

Thank you for contacting us.

Regarding ab62623, Stressed tissue such as Alzheimer's affected neurons. or Human brain pre treated with 10ug proteinase K for 40mins at 37oC are suitable positive controls.

Regarding ab23722, human colon ...

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Thank you for your reply.

Could you please confirm the order number so I can raise the new free order for ab62623?

We appreciate any feedback from customers using our products. The suggested tips aimed to help you solve the problems...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Sep 11 2012

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"