Anti-8 Hydroxyguanosine antibody [7D7E4] (ab26842)


  • Product nameAnti-8 Hydroxyguanosine antibody [7D7E4]
    See all 8 Hydroxyguanosine primary antibodies
  • Description
    Mouse monoclonal [7D7E4] to 8 Hydroxyguanosine
  • Specificityab26842 recognizes the oxidized epitope (8-OH dG) and not the 2'-deoxyguanosine (dG)
  • Tested applicationsSuitable for: ELISA, IHC-P, ICC/IFmore details
  • Immunogen

    8-hydroxy-2'deoxyguanisine conjugated to carrier protein.

  • Positive control
    • Hamster oral epithelial cancerous tissue.



Our Abpromise guarantee covers the use of ab26842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000. For detecting 0.1ng of 8-OHdG-BSA.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.


  • Relevance8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease. 8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress.
  • Alternative names
    • 8 hydroxy 2’ deoxyguanosine antibody
    • 8 hydroxyguanine antibody
    • 8 hydroxyguanosine antibody
    • 8 OHG antibody
    • 8-OHG antibody
    • 8OG antibody
    • 8OHdG antibody
    • 8OHG antibody
    see all

Anti-8 Hydroxyguanosine antibody [7D7E4] images

  • Testing ab26842 8-OHdG mouse monoclonal antibody with competitive ELISA using dA, dT, dC and dG as competitive antigen.

  • ab26842 at 1/50 dilution, staining 8 Hydroxyguanosine in hamster oral epithelial cancerous tissue, by Immunohistochemistry. The image shows that that ab26842 can stain DNA damage in the cytoplasm and the nucleus.
  • Immunohistochemical analysis of pig esophageal tissue, staining 8 Hydroxyguanosine with ab26842. ab97240 - Goat polyclonal to Mouse IgG1 - heavy chain (HRP) was used as the secondary antibody.

References for Anti-8 Hydroxyguanosine antibody [7D7E4] (ab26842)

This product has been referenced in:
  • Riveiro-Naveira RR  et al. Resveratrol lowers synovial hyperplasia, inflammatory markers and oxidative damage in an acute antigen-induced arthritis model. Rheumatology (Oxford) 55:1889-900 (2016). Read more (PubMed: 27354682) »
  • Barrett CW  et al. Dietary selenium deficiency exacerbates DSS-induced epithelial injury and AOM/DSS-induced tumorigenesis. PLoS One 8:e67845 (2013). ICC/IF . Read more (PubMed: 23861820) »

See all 5 Publications for this product

Product Wall

As indicated on the datasheet, the experimental settings require an assay dependent dilution, which needs to be found for every individual test series. On the other hand, this also means that this product is quite robust and does not need a "speci...

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Thank you for contacting us.

My Spanish speaking colleague is currently out of the office, unfortunately my Spanish is not good enough to write my reply in your language.

As stated on the product datasheets, both anti-8 Hydroxyguanosi...

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Wehaven’t tested for cross-reactivity to ketone formation.

I'm sorry to not be able to provide further information in this instance. Please contact me again if you have any further questions.

As this antibody detects a biochemical and not a protein, there shouldn't be any species restriction forthis antibody.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Meriones unguiculatus Tissue sections (stomach)
Specification stomach
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer (10mM, pH 6)
Permeabilization Yes - Dako wash buffer
Blocking step Dako peroxidase Block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 22 2012

Thank you for your telephone call yesterday afternoon.

I am sorry that the vial you received did not contain the full amount of the product and I apologize for the inconvenience.

As requested, I have issued a free of charge replac...

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Thank you for your enquiry.

Both these antibodies are designed to detect a specific DNA modification which is a species-independent reactivity. So yes, both antibodies are suitable for use in rat.

I hope this is helpful. Please cont...

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Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact ...

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Thank you for your reply.
I have still not heard back from all of those products. I am going to send email reminders and follw up with what is happening. I will get back to you as soon as I find out anything.
Thank you for your patience.

Thank you for getting back to me and for confirming the order details. It is very much appreciated.

This is to let you know that I have placed a new order for you - for one vial ofab48508 as a free of charge replacement (exchange f...

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