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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides
Anti-8 Hydroxyguanosine antibody
See all 8 Hydroxyguanosine products (5) ...
Goat polyclonal to 8 Hydroxyguanosine
ab10802 cross reacts completely with 8-OHdG,and does not cross react with other naturally occurring nucleotides.
IHC-P, ELISA, ICC/IFmore details
Due to the conserved nature of the target reactivity would be predicted in wide range of species.
8-Hydroxyguanosine - conjugate.
Stressed tissue such as Alzheimer's affected neurons. or Human brain pre treated with 10ug proteinase K for 40mins at 37oC
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Whole Serum
Whole antiserum
Polyclonal
IgG
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 8 Hydroxyguanosine antibody (ab10802)
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Our Abpromise guarantee covers the use of ab10802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/200
ELISA: 1/100000 - 1/250000.
ICC/IF: Use at an assay dependent dilution. (PubMed: 16678792)
8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease. 8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 8 Hydroxyguanosine antibody (ab10802)

IHC staining on Alzheimer disease brain showing oxidized RNA in neurons. The tissue sections were formalin-fixed, paraffin embedded with either (A) no pretreatment or (B) pretreatment with 10 µg/ml proteinase K for 40 minutes at 37°C. Each was stained with a 1:200 dilution.
This product has been referenced in:
See all 2 publications for this product
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IHC staining on Alzheimer disease brain showing oxidized RNA in neurons. The tissue sections were formalin-fixed, paraffin embedded with either (A) no pretreatment or (B) pretreatment with 10 µg/ml proteinase K for 40 minutes at 37°C. Each was stained with a 1:200 dilution.
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