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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 8 Hydroxyguanosine antibody (ab10802)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 8 Hydroxyguanosine antibody (ab10802)
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Product Name 

8 Hydroxyguanosine antibody

 

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Product type 

Primary antibodies

Description 

Goat polyclonal to 8 Hydroxyguanosine

Immunogen 

8-Hydroxyguanosine - conjugate.

Species Reactivity 

Due to the conserved nature of the target reactivity would be predicted in wide range of species.

Specificity 

ab10802 cross reacts completely with 8-OHdG,and does not cross react with other naturally occurring nucleotides.

Tested applications 
(see key)

ELISA, IHC-P


Abreviews 

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Application notes 
(see key)

Recommended dilutions
ELISA: 1/100000 - 1/250000.
IHC-P: 1/200.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Stressed tissue such as Alzheimer's affected neurons.
or
Human brain pre treated with 10ug proteinase K for 40mins at 37oC

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides

Relevance 

8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease.

8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress.

 

Alternative names 8 Hydroxyguanosine antibody (ab10802)

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: Whole Serum

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this 8 Hydroxyguanosine antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for 8 Hydroxyguanosine antibody images, references, products related to ab10802 and other tools.

 

8 Hydroxyguanosine antibody images:

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 8 Hydroxyguanosine antibody (ab10802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 8 Hydroxyguanosine antibody (ab10802)

IHC staining on Alzheimer disease brain showing oxidized RNA in neurons. The tissue sections were formalin-fixed, paraffin embedded with either (A) no pretreatment or (B) pretreatment with 10 µg/ml proteinase K for 40 minutes at 37°C. Each was stained with a 1:200 dilution.

 
 

Search PubMed (MEDLINE) for references to 8 Hydroxyguanosine

 

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