Overview

  • Product name
    Anti-8 Hydroxyguanosine antibody
    See all 8 Hydroxyguanosine primary antibodies
  • Description
    Goat polyclonal to 8 Hydroxyguanosine
  • Specificity
    ab10802 cross reacts completely with 8-OHdG,and does not cross react with other naturally occurring nucleotides.
  • Tested applications
    Suitable for: IHC-P, ELISAmore details
  • Species reactivity
    Due to the conserved nature of the target reactivity would be predicted in wide range of species.
  • Immunogen

    8-Hydroxyguanosine - conjugate.

  • Positive control
    • Stressed tissue such as Alzheimer's affected neurons. or Human brain pre treated with 10ug proteinase K for 40mins at 37oC

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Whole Serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.

Perform enzymatic antigen retrieval with 10 µg/ml proteinase K for 40 minutes at 37°C.

ELISA 1/100000 - 1/250000.

Target

  • Relevance
    8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease. 8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress.
  • Alternative names
    • 8 hydroxy 2’ deoxyguanosine antibody
    • 8 hydroxyguanine antibody
    • 8 hydroxyguanosine antibody
    • 8 OHG antibody
    • 8-OHG antibody
    • 8OG antibody
    • 8OHdG antibody
    • 8OHG antibody
    see all

Images

  • Immunohistochemical analysis of cat hepatic tissue, labeling 8 Hydroxyguanosine with ab10802. Sample fixed in paraformaldehyde. Heat mediated antigen retrieval with Tris-EDTA (pH9). Treated with ab10802 diluted 1/1500 in PBS plus casein for 1 hour and 30 minutes at 37°C. Secondary was ImmPress anti-goat HRP-conjugated polyclonal antibody.

    See Abreview

  • IHC staining on Alzheimer disease brain showing oxidized RNA in neurons. The tissue sections were formalin-fixed, paraffin embedded with either (A) no pretreatment or (B) pretreatment with 10 µg/ml proteinase K for 40 minutes at 37°C. Each was stained with a 1:200 dilution.

References

This product has been referenced in:
  • Snyder B  et al. Chronic intermittent hypoxia induces oxidative stress and inflammation in brain regions associated with early-stage neurodegeneration. Physiol Rep 5:N/A (2017). IHC-FoFr ; Rat . Read more (PubMed: 28473320) »
  • Marazita MC  et al. Oxidative stress-induced premature senescence dysregulates VEGF and CFH expression in retinal pigment epithelial cells: Implications for Age-related Macular Degeneration. Redox Biol 7:78-87 (2016). Read more (PubMed: 26654980) »

See all 14 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In or...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Mouse Tissue (colon)
Specification
colon
Username

Dr. K Kulmira Nurgali

Verified customer

Submitted Nov 10 2017

This product has not been purified and is sold as a whole antiserum therefore we have not determined its concentration. As a rough guideline, in general whole antiserum has a concentration of 1-10 mg/ml.

The antibody will detect 8-OHdG in DNA and also RNA. RNase digestion recommended if you want to detect only 8-OHdG in DNA.

The paper at the following link used a different antibody, a mouse monoclonal antibody, but the procedure for staining ...

Read More

The immunostain that used the antibody at 1/4000 used a biotin-conjugated secondary, so it is likely that this was followed with an avidin-biotin-HRP complex amplification. It is possible that 1/4000 was in fact effective, as antibody affinities vary a...

Read More

Are you confident the secondary antibody and other reagents are effective? If so, I agree that it is best to try a different antibody at this point, which I will send free-of-charge.

Abcam has several others against 8-Hydroxyguanosine but as...

Read More

Thank you for your enquiry.

I can confirm that ab10802 Anti-8 Hydroxyguanosine antibody is tested as suitable for indirect ELISA.

However, I am sorry to confirm that as far as we are aware, ab10802 has never been tested in flow cy...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (liver)
Specification
liver
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA (pH 9.0)
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 04 2013

Thank you for contacting us.

My Spanish speaking colleague is currently out of the office, unfortunately my Spanish is not good enough to write my reply in your language.

As stated on the product datasheets, both anti-8 Hydroxyguanosi...

Read More

I appreciate the time taken to submit further information to us.

Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applicatio...

Read More

1-10 of 22 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up