Anti-8 Hydroxyguanosine antibody (ab93295)

Overview

  • Product name
    Anti-8 Hydroxyguanosine antibody
    See all 8 Hydroxyguanosine primary antibodies
  • Description
    Goat polyclonal to 8 Hydroxyguanosine
  • Specificity
    ab93295 detects 8 Hydroxyguanosine in synthetic or other in vitro samples.
  • Tested applications
    Suitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Not applicable
  • Immunogen

    8 Hydroxyguanosine conjugate

Properties

Applications

Our Abpromise guarantee covers the use of ab93295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IHC-P
  • Application notes
    ELISA: 1/100000.
    IHC-P: 1/200.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease. 8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress.
    • Alternative names
      • 8 hydroxy 2’ deoxyguanosine antibody
      • 8 hydroxyguanine antibody
      • 8 hydroxyguanosine antibody
      • 8 OHG antibody
      • 8-OHG antibody
      • 8OG antibody
      • 8OHdG antibody
      • 8OHG antibody
      see all

    References

    ab93295 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 25°C
    Antigen retrieval step
    Heat mediated
    Sample
    Mouse Tissue sections (Lung)
    Specification
    Lung
    Permeabilization
    Yes - 0.01% triton x
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 29 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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