Anti-8 Hydroxyguanosine antibody [N45.1] (ab48508)


  • Product name
    Anti-8 Hydroxyguanosine antibody [N45.1]
    See all 8 Hydroxyguanosine primary antibodies
  • Description
    Mouse monoclonal [N45.1] to 8 Hydroxyguanosine
  • Specificity
    Nineteen analogues of 8-Hydroxy-2'-deoxyguanosine {guanosine (G), 7-methyl-G, 6-SH-G, 8-bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine (Gua), O6-methyl-Gua, 8-OHGua, uric acid, Urea, creatine, creatinine} demonstrate no cross-reactivity. Only 8-sulfhydryl-G and 8-OHG demonstrate minimal cross-reactivity (less than 1%).
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Immunogen

    Chemical/ Small Molecule: 8-Hydroxy-2'-deoxyguanosine conjugated Keyhole Limpet Hemocyanin

  • Positive control
    • Epidermis from hairless mouse treated for 4 weeks with chronic UVB irradiation, or kidney treated with Fe-NTA.



Our Abpromise guarantee covers the use of ab48508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

For mouse on mouse staining we recommend uisng MOM kit - ab127055


  • Relevance
    8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease. 8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress.
  • Alternative names
    • 8 hydroxy 2’ deoxyguanosine antibody
    • 8 hydroxyguanine antibody
    • 8 hydroxyguanosine antibody
    • 8 OHG antibody
    • 8-OHG antibody
    • 8OG antibody
    • 8OHdG antibody
    • 8OHG antibody
    see all


  • De-paraffinized and dehydrated sections were treated in a microwave oven at low power for 10 min in 0.01 M sodium citrate buffer (pH 6.0) to retrieve antigen, and inactivated endogenous peroxidase using 3% hydrogen peroxide in methanol for 10 min, and blocked with blocking solution for 1 hour at room temperature. Sections were then incubated with 1∶20 dilution of mouse monoclonal antibody to 8-hydroxy-2′-deoxyguanosine (8OHdG) (ab48508) overnight at 4°C. For detection, a biotinylated anti-mouse and HRP-labeled Streptavidin, and staining with DAB was used.

    Immunohistochemical staining of nitrotyrosine (A) and 8-hydroxy-2′-deoxyguanosine (B) was performed on 5 rats randomly chosen from each group (ND: chow diet group, HFD: High fat diet, HFD-QH: High fat diet treated with Qing Huo Yi Hao" (QHYH)). An islet is encircled in each quandrant. Black arrows point to islets of Langerhans (Original magnification ×200). Percentage of immunohistochemial positive staining area in islet out of the area of corresponding islet (C, D) was presented. Values and bars represent means±SEM. * and ** denote P<0.05 and P<0.001 versus group ND.

  • ab48508 staining 8 Hydroxyguanosine in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a TE buffer. Samples were incubated with primary antibody (1/50) for 30 mins at 20°C. A HRP-conjugated Goat anti-mouse IgG was used as the secondary antibody.

    See Abreview

  • Ab48508, at a concentration of 5 µg/ml, staining 8-Hydroxy-2'-deoxyguanosine in epidermis from hairless mouse after 4 weeks of treatment by chronic UVB irradiation.
  • ab48508 staining 8-Hydroxy-2'-deoxyguanosine in human spermatozoa by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using triton in 4% BSA for 15 minutes at room temperature, blocked with 4% BSA for 30 minutes at 20°C and then incubated using ab48508 at a 1/200 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 350 conjugated goat anti-mouse monoclonal, used at a 1/250 dilution.

    Staining from left to right: 1. TUNEL 2. ab48508 3. DIC 4. Merge.

    See Abreview


This product has been referenced in:
  • Jia YB  et al. Inhibitory effects of vitamin E on osteocyte apoptosis and DNA oxidative damage in bone marrow hemopoietic cells at early stage of steroid-induced femoral head necrosis. Mol Med Rep 15:1585-1592 (2017). Read more (PubMed: 28259972) »
  • Zeng J  et al. Isoliquiritigenin alleviates early brain injury after experimental intracerebral hemorrhage via suppressing ROS- and/or NF-?B-mediated NLRP3 inflammasome activation by promoting Nrf2 antioxidant pathway. J Neuroinflammation 14:119 (2017). IHC-P ; Rat . Read more (PubMed: 28610608) »

See all 25 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Frozen sections)
Mouse Tissue sections (Liver)
Yes - 0.3%triton-X
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Dr. Yoshihisa Koyama

Verified customer

Submitted Dec 22 2017

Thank you for your enquiry regarding ab48508.

Would you be so kind to confirm if the tube has been centrifuged before opening the lid? It would help me if you could specify how much volume of the liquid was in the vial?

Thank you fo...

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Thank you for contacting us.

I can recommend the antibody ab48508 against 8-hydroxy-2'-deoxyguanosine. This antibody has been tested in immunohistochemistry on paraffin embedded tissue sections (IHC-P).

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I am writing to you as I have been informed by our logistic group that ourAnti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1], AB48508, now ships with a concentration of 1.0mg/ml instead of 0.1mg/ml. As such the aliquot you receive will contain 50ug in a...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not parrive with the volume as stated on the datasheet and for the inconvenie...

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Thank you for your enquiry.

Both these antibodies are designed to detect a specific DNA modification which is a species-independent reactivity. So yes, both antibodies are suitable for use in rat.

I hope this is helpful. Please cont...

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I have heard back about ab48508.
The antibody ab48508 (anti 8-OHdG monoclonal antibody) is specific for
8-Hydroxy-2'-deoxyguanosine(8-OHdG). 8-Hydroxyguanosine (8OHG) demonstrate
minimal crossreactivity (less than 1%).
I will get ba...

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Thank you for your reply.
I have still not heard back from all of those products. I am going to send email reminders and follw up with what is happening. I will get back to you as soon as I find out anything.
Thank you for your patience.

Thank you for getting back to me and for confirming the order details. It is very much appreciated.

This is to let you know that I have placed a new order for you - for one vial ofab48508 as a free of charge replacement (exchange f...

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Thank you for your response.

The replacement vial will be free of charge so you do nothave to pay for it. Could you please confirm the Abcam Order Number and the delivery address so that I can place a new order for you as soon as possible? Read More

1-10 of 16 Abreviews or Q&A


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