Overview

  • Product name8 isoprostane ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 12 0.43%
    Inter-assay
    Sample n Mean SD CV%
    Overall 12 1.75%
  • Sample type
    Urine, Serum, Plasma, Cell culture extracts, Tissue
  • Assay typeCompetitive
  • Sensitivity
    1 pg/ml
  • Range
    0.005 ng/ml - 5 ng/ml
  • Assay durationMultiple steps standard assay
  • Product overview

    Abcam’s 8 isoprostane competitive in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the determination of 8 isoprostane levels in biological samples. The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissues phospholipids by oxygen radicals. A recent NIH-sponsored study on Biomarkers of Oxidative Stress has indicated that 8 isoprostane is the best index of oxidative injury in a well-accepted oxidant stress rat model. In addition, plasma 8 isoprostane levels were found to be elevated in elderly subjects with severe hypertension and in urine from subjects with high fat diet-induced liver steatosis.

    This kit can be used for the determination of 8 isoprostane in diluted urine, serum, plasma, cells and tissues following proper isolation and purification of the eicosanoid from the isoprostane-containing sample.

     

  • Tested applicationsSuitable for: Competitive ELISAmore details
  • PlatformMicroplate

Properties

  • Alternative names
    • Prostaglandin, iso Prostaglandin F2, iso Prostaglandin, iPF2 alpha

Applications

Our Abpromise guarantee covers the use of ab175819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

8 isoprostane ELISA Kit images

  • Example of standard curve obtained with ab175819.

Protocols

References for 8 isoprostane ELISA Kit (ab175819)

ab175819 has not yet been referenced specifically in any publications.

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The best places to stop are after drying the ethyl acetate layer. The sample can be stored at minus twenty degrees for an extensive period of time.



I hope this informatio...

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This kit can be used with any species as the isoprostane is a well conserved compound throughout species.

The 96-well plate is coated with anti 8-isoprostane antibody in each well, which will capture the samples 8 isoprostane to be m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"