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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Overview
Product name
Anti-A RAF antibody
See all A RAF products (6) ...
Description
Mouse monoclonal to A RAF
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: RQQFYHSVQD LSGGSRQHEA PSNRPLNELL TPQGPSPRTQ HCDPEHFPFP APANAPLQRI RSTSTPNVHM VSTTAPMDSN LIQLTGQSFS TDAAGSRGGS , corresponding to amino acids 151-251 of Human A RAF
RQQFYHSVQD LSGGSRQHEA PSNRPLNELL TPQGPSPRTQ HCDPEHFPFP APANAPLQRI RSTSTPNVHM VSTTAPMDSN LIQLTGQSFS TDAAGSRGGS
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
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Western blot - A RAF antibody (ab54827)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
Tissue specificity
Predominantly in urogenital tissues.
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain.
Target information above from: UniProt accessionP10398
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A raf 1 antibody
- A Raf proto oncogene serine/threonine protein kinase antibody
- ARAF 1 antibody
see all
Database links
- Entrez Gene: 369 Human
- Omim: 311010 Human
- SwissProt: P10398 Human
- Unigene: 446641 Human
Anti-A RAF antibody images:
Western blot - A RAF antibody (ab54827)
A RAF antibody (ab54827) at 1ug/lane + HeLa cell lysate at 25ug/lane.
References for Anti-A RAF antibody (ab54827)
ab54827 has not yet been referenced specifically in any publications.
Publishing research using ab54827? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"