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All lanes : Anti-A RAF antibody (ab59343) at 1/500 dilution
Lane 1 : Extracts from HeLa cells treated
with PMA (125ng/ml, 30mins)
Lane 2 : Extracts from HeLa cells treated
with PMA (125ng/ml, 30mins), plus immunising peptide
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
ab59343, at a 1/50-1/100 dilution, staining human A RAF in breast carcinoma with (+) or without (-) immunising peptide), using Immunohistochemistry, Paraffin embedded tissue
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