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Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Overview
Product name
Anti-A RAF antibody
See all A RAF products (6) ...
Description
Rabbit polyclonal to A RAF
Specificity
ab59343 detects endogenous levels of total A RAF protein.
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthesized non-phosphopeptide derived from human A RAF around the phosphorylation site of tyrosine 301/tyrosine 302 (G-Y-YP-W-E).
Positive control
Human breast carcinoma tissue Extracts from HeLa cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 68 kDa.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/20000ELISA: 1/20000
Target
Function
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
Tissue specificity
Predominantly in urogenital tissues.
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain.
Target information above from: UniProt accessionP10398
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A raf 1 antibody
- A Raf proto oncogene serine/threonine protein kinase antibody
- ARAF 1 antibody
see all
Database links
- Entrez Gene: 369 Human
- Entrez Gene: 11836 Mouse
- Entrez Gene: 64363 Rat
- Omim: 311010 Human
- SwissProt: P10398 Human
- SwissProt: P04627 Mouse
- SwissProt: P14056 Rat
- Unigene: 446641 Human
see all
Anti-A RAF antibody images:
Western blot - A RAF antibody (ab59343)
All lanes : Anti-A RAF antibody (ab59343) at 1/500 dilution
Lane 1 : Extracts from HeLa cells treated
with PMA (125ng/ml, 30mins)
Lane 2 : Extracts from HeLa cells treated
with PMA (125ng/ml, 30mins), plus immunising peptide
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - A RAF antibody (ab59343)
ab59343, at a 1/50-1/100 dilution, staining human A RAF in breast carcinoma with (+) or without (-) immunising peptide), using Immunohistochemistry, Paraffin embedded tissue
References for Anti-A RAF antibody (ab59343)
ab59343 has not yet been referenced specifically in any publications.
Publishing research using ab59343? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"