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All lanes : Anti-A RAF (phospho Y301 + Y302) antibody (ab59439) at 1/500 dilution
Lane 1 : HeLa cell extract, treated with PMA (125ng/ml, 30 mins)
Lane 2 : HeLa cell extract, treated with PMA (125ng/ml, 30 mins) with phosphopeptide
Predicted band size : 68 kDa
Observed band size : 68 kDa
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