You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-A RAF (phospho Y301 + Y302) antibody (ab59439)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-A RAF (phospho Y301 + Y302) antibody
See all A RAF products (6) ...

Description

Rabbit polyclonal to A RAF (phospho Y301 + Y302)

Specificity

Detects endogenous levels of A RAF only when phosphorylated at tyrosine 301/tyrosine 302.

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic phosphopeptide (Human) from around the phosphorylation site of tyrosine 301/tyrosine 302 (GYYPWE) of A RAF.

Positive control

HeLa cell extract treated with PMA (125ng/ml, 30mins).

General notes

Phosphorylation sites: Human: Tyr301/Tyr302; Mouse: Tyr299/Tyr300; Rat: Tyr299/Tyr300

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases

  • Western blot - A RAF (phospho Y301 + Y302) antibody (ab59439)Western blot - A RAF (phospho Y301 + Y302) antibody (ab59439) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab59439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).

  • ELISA

     ELISA: 1/20000

    ELISA: 1/20000

Target

Function

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.

Tissue specificity

Predominantly in urogenital tissues.

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain.

Target information above from: UniProt accessionP10398 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • A raf 1 antibody
  • A Raf proto oncogene serine/threonine protein kinase antibody
  • ARAF 1 antibody
  • ARAF antibody
  • ARaf proto oncogene serine/threonine protein kinase antibody
  • ARAF_HUMAN antibody
  • ARAF1 antibody
  • Oncogene Araf1 antibody
  • Oncogene PKS2 antibody
  • PKS 2 antibody
  • PKS antibody
  • PKS2 antibody
  • Proto oncogene Pks antibody
  • Proto-oncogene A-Raf antibody
  • Proto-oncogene A-Raf-1 antibody
  • Proto-oncogene Pks antibody
  • RAFA 1 antibody
  • RAFA1 antibody
  • Ras binding protein DA Raf antibody
  • Serine/threonine-protein kinase A-Raf antibody
  • v raf murine sarcoma 3611 viral oncogene homolog antibody
  • v raf murine sarcoma 3611 viral oncogene homolog 1 antibody
  • v raf oncogene homolog 1 (murine sarcoma 3611 virus) antibody
see all

Database links

see all

Anti-A RAF (phospho Y301 + Y302) antibody images:

  Western blot - A RAF (phospho Y301 + Y302) antibody (ab59439)

Western blot - A RAF (phospho Y301 + Y302) antibody (ab59439)

All lanes : Anti-A RAF (phospho Y301 + Y302) antibody (ab59439) at 1/500 dilution

Lane 1 : HeLa cell extract, treated with PMA (125ng/ml, 30 mins)
Lane 2 : HeLa cell extract, treated with PMA (125ng/ml, 30 mins) with phosphopeptide


Predicted band size : 68 kDa
Observed band size : 68 kDa

References for Anti-A RAF (phospho Y301 + Y302) antibody (ab59439)

ab59439 has not yet been referenced specifically in any publications.

Publishing research using ab59439? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"