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A RAF protein (Active) (ab79795)

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Overview

Product name

A RAF protein (Active)

Protein description

Recombinant fragment, corresponding to amino acids 282-606 of Human A RAF, containing the mutations YY301-302DD, with an N-terminal proprietary tag, expressed in a Baculovirus infected Sf9 cell expression system, 63.6 kDa (SwissProt ID P10398).

Properties

Purification notes

Purity is 80% by SDS-PAGE.

Biological activity

Specific Activity: 50 nM A RAF yields 8% conversion of 1 uM ATP using MEK1 (K97R) as substrate.

Form

Liquid

Storage instructions

Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

Concentration

Concentration information loading...

Research areas

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases

  • SDS-PAGE - A RAF protein (Active) (ab79795)SDS-PAGE - A RAF protein (Active) (ab79795) image (enlarge)

Applications

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Our Abpromise guarantee covers the use of ab79795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Protein info

Function

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.

Tissue specificity

Predominantly in urogenital tissues.

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain.

Target information above from: UniProt accessionP10398 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

A RAF protein (Active) images:

  SDS-PAGE - A RAF protein (Active) (ab79795)

SDS-PAGE - A RAF protein (Active) (ab79795)

10% SDS-PAGE showing ab79795 at approximately 63.6kDa (3µg).

References for A RAF protein (Active) (ab79795)

ab79795 has not yet been referenced specifically in any publications.

Publishing research using ab79795? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"