Feedback Form

SDS-PAGE - A RAF protein (Active) (ab79795)
SDS-PAGE - A RAF protein (Active) (ab79795)
(enlarge)


Product Name 

A RAF protein (Active)

Product type 

Proteins and Peptides

Peptide 

Recombinant fragment, corresponding to amino acids 282-606 of Human A RAF, containing the mutations YY301-302DD, with an N-terminal proprietary tag, expressed in a Baculovirus infected Sf9 cell expression system, 63.6 kDa (SwissProt ID P10398).

Specificity 

Specific Activity: 50 nM A RAF yields 8% conversion of 1 uM ATP using MEK1 (K97R) as substrate.

Tested applications 
(see key)

FuncS, SDS-PAGE

Application notes 
(see key)

Recommended dilutions
FuncS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases

Relevance 

The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.

 

Alternative names A RAF protein (Active) (ab79795)

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Purification notes 

Purity is 80% by SDS-PAGE.

Form 

Liquid

Storage instructions 

Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this A RAF protein (Active) is on this datasheet. But please do contact us if you would like any reassurance!


 

A RAF protein (Active) images:

Product image  SDS-PAGE - A RAF protein (Active) (ab79795)

SDS-PAGE - A RAF protein (Active) (ab79795)

10% SDS-PAGE showing ab79795 at approximately 63.6kDa (3µg).

 
 

Search PubMed (MEDLINE) for references to A RAF

 

A RAF protein (Active) - more information

Customer reviews (feedback) regarding A RAF protein (Active)

Customer FAQs regarding A RAF protein (Active)

Protocols for A RAF protein (Active)

Price and availability of products related to A RAF protein (Active)

 

A RAF protein (Active) - other tools:

Contact Abcam with a Technical Enquiry about A RAF protein (Active)

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.