|
|
|
|
SDS-PAGE - A RAF protein (Active) (ab79795)
(enlarge)
|
|
|
|
|
Product Name
|
A RAF protein (Active)
|
|
Product type
|
Proteins and Peptides
|
|
Peptide
|
Recombinant fragment, corresponding to amino acids 282-606 of Human A RAF, containing the mutations YY301-302DD, with an N-terminal proprietary tag, expressed in a Baculovirus infected Sf9 cell expression system, 63.6 kDa (SwissProt ID P10398).
|
|
Specificity
|
Specific Activity: 50 nM A RAF yields 8% conversion of 1 uM ATP using MEK1 (K97R) as substrate.
|
|
Tested applications
(see key)
|
FuncS, SDS-PAGE
|
|
Application notes
(see key)
|
Recommended dilutions
FuncS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Research areas
|
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Signal transducers
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Kinases
|
|
Relevance
|
The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.
|
|
|
|
|
Storage buffer
|
Preservative: None
Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0 Material safety datasheet (MSDS) for this product:
Glycerol MSDS
|
|
Purification notes
|
Purity is 80% by SDS-PAGE.
|
|
Form
|
Liquid
|
|
Storage instructions
|
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this A RAF protein (Active) is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
A RAF protein (Active) images:
|
 SDS-PAGE - A RAF protein (Active) (ab79795)
10% SDS-PAGE showing ab79795 at approximately 63.6kDa (3µg).
|
| |
| |
|
Search PubMed (MEDLINE) for references to A RAF
|
| |
A RAF protein (Active) - more information
|
Customer reviews (feedback) regarding A RAF protein (Active) |
Customer FAQs regarding A RAF protein (Active) |
Protocols for A RAF protein (Active) |
Price and availability of products related to A RAF protein (Active) |
| |
A RAF protein (Active) - other tools:
|
Contact Abcam with a Technical Enquiry about A RAF protein (Active) |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
