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Overview

  • Product nameA RAF protein (Tagged)See all A RAF proteins and peptides ...
  • Protein descriptionRecombinant full length Human A RAF protein with an N terminal proprietary tag.
  • Uniprot accessionQ96II5
  • Molecular weight92.620kDa inclusive of tags
  • Protein length609 amino acids
  • Expression hostWheat germ

    • Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl
  • Concentration information loading...
  • Additional notesProtein concentration is above or equal to 0.05 mg/ml.
    This protein is best used within three months from the date of receipt.
  • Sequence notesMEPPRGPPANGAEPSRAVGTVKVYLPNKQRTVVTVRDGMS VYDSLDKALKVRGLNQDCCVVYRLIKGRKTVTAWDTAIAP LDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLF HGFRCQTCGYKFHQHCSSKVPTVCVDMSTNRQQPSRFYHS VQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHF PFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQ SFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAE QRERKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKRIG TGSFGTVFRGRWHGDVAVKVLKVSQPTAEQAQAFKNEMQV LRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVA DTRFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFL HEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEV IRMQDPNPYSFQSDVYAYGVVLYELMTGSLPYSHIGCRDQ IIFMVGRGYLSPDLSKISSNCPKAMRRLLSDCLKFQREER PLFPQILATIELLQRSLPKIERSASEPSLHRTQADELPAC LLSAARLVP
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab132213 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA ELISA: Use at an assay dependent concentration.
    SDS-PAGE SDS-PAGE: Use at an assay dependent concentration.
    WB WB: Use at an assay dependent concentration. (Recombinant protein)
  • Application notesThis peptide can be used with studies using ab170253.

    • Protein info

    • Alternative names
        A raf 1A Raf proto oncogene serine/threonine protein kinaseAraf
        ARAF 1ARaf proto oncogene serine/threonine protein kinaseARAF_HUMANARAF1Oncogene Araf1Oncogene PKS2PKSPKS 2PKS2Proto oncogene PksProto-oncogene A-RafProto-oncogene A-Raf-1Proto-oncogene PksRAFA 1RAFA1Ras binding protein DA RafSerine/threonine-protein kinase A-Rafv raf murine sarcoma 3611 viral oncogene homologv raf murine sarcoma 3611 viral oncogene homolog 1v raf oncogene homolog 1 (murine sarcoma 3611 virus)
      see all
  • FunctionInvolved in the transduction of mitogenic signals from the cell membrane to the nucleus.
  • Tissue specificityPredominantly in urogenital tissues.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 RBD (Ras-binding) domain.

    • Target information above from: UniProt accession P10398 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    A RAF protein (Tagged) images

    • SDS-PAGE - A RAF protein (Tagged) (ab132213)
      SDS-PAGE - A RAF protein (Tagged) (ab132213)
      12.5% SDS-PAGE analysis of ab132213 stained with Coomassie Blue.

    References for A RAF protein (Tagged) (ab132213)

    ab132213 has not yet been referenced specifically in any publications.

    Publishing research using ab132213 ? Please let us know so that we can cite the reference in this datasheet

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