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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-A1CF antibody
See all A1CF products (4) ...
Description
Rabbit polyclonal to A1CF
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (EAVCLGTCPE PEASMSTAIP GLKKGNNALQ SIILQTLLEK ENGQRKYGGP) of human A1CF (NP_620311).
EAVCLGTCPE PEASMSTAIP GLKKGNNALQ SIILQTLLEK ENGQRKYGGP
Positive control
HepG2 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified by peptide affinity chromatography method.
Clonality
Polyclonal
Isotype
IgG
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Western blot - A1CF antibody (ab83276)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay, 1:1562500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay.
Tissue specificity
Widely expressed with highest levels in brain, liver, pancreas, colon and spleen.
Sequence similarities
Contains 3 RRM (RNA recognition motif) domains.
Domain
The RRM domains are necessary but not sufficient for binding to APOB mRNA. Additional residues in the pre-RRM and C-terminal regions are required for RNA-binding and for complementing APOBEC1 activity.
Cellular localization
Nucleus. Endoplasmic reticulum. Cytoplasm. Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum (By similarity). Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1.
Target information above from: UniProt accessionQ9NQ94
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- A1CF antibody
- A1CF_HUMAN antibody
- ACF antibody
see all
Database links
- Entrez Gene: 29974 Human
- SwissProt: Q9NQ94 Human
- Unigene: 499643 Human
Anti-A1CF antibody images:
Western blot - A1CF antibody (ab83276)
Anti-A1CF antibody (ab83276) at 1 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 65 kDa
Observed band size : 65 kDa
References for Anti-A1CF antibody (ab83276)
ab83276 has not yet been referenced specifically in any publications.
Publishing research using ab83276? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"