If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab89050 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
|
|||||||||
I have used ab89050 for western blotting of protein from 60ug liver, which is a tissue that expresses high levels of A1CF. Using the recommended dilution of 1:500 did not produce any bands but using 1:100 did produce the band at the expected molecular weight. However this is an unnacceptably high amount of antibody to use. |
|||||||||
ANSWER: |
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab89050. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research. |
||||||||
|
|||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-A1CF antibody (ab89050) at 1 µg/ml + Human placenta lysate at 50 µg
Predicted band size : 65 kDa
Anti-A1CF antibody (ab89050) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size : 65 kDa
All lanes : Anti-A1CF antibody (ab89050) at 1 µg/ml
Lane 1 : A1CF-transfected 293T cell lysate
Lane 2 : non transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 65 kDa
Observed band size : 65 kDa
0
Call 01223 696 000 or contact us