Anti-A1CF antibody (ab89050)

Overview

• Product nameAnti-A1CF antibodySee all A1CF primary antibodies ...
• Description
  Mouse polyclonal to A1CF
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Full length protein, corresponding to amino acids 1-594 of Human A1CF (NP_620311.1)

• Positive controlPlacenta or HeLa cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing
  • Other

  • Epigenetics and Nuclear Signaling
  • Chromatin Binding Proteins
  • DNA / RNA binding

Applications

Target

• FunctionEssential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay.
• Tissue specificityWidely expressed with highest levels in brain, liver, pancreas, colon and spleen.
• Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
• DomainThe RRM domains are necessary but not sufficient for binding to APOB mRNA. Additional residues in the pre-RRM and C-terminal regions are required for RNA-binding and for complementing APOBEC1 activity.
• Cellular localizationNucleus. Endoplasmic reticulum. Cytoplasm. Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum (By similarity). Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1.

Target information above from: UniProt accession Q9NQ94 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 29974 Human
  • SwissProt: Q9NQ94 Human
  • Unigene: 282795 Human

• Alternative names
  A1CF antibodyA1CF_HUMAN antibodyACF antibody

  ACF64 antibodyACF65 antibodyApo B RNA editing protein antibodyApobec 1 complementation factor antibodyApobec 1 complementation factor (ACF) (ASP) antibodyAPOBEC 1 stimulating protein antibodyAPOBEC1 complementation factor antibodyAPOBEC1 stimulating protein antibodyAPOBEC1-stimulating protein antibodyAPOBEC1CF antibodyASP antibodyMGC163391 antibodyRP11-564C4.2 antibody

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Anti-A1CF antibody images

• 

  Western blot - A1CF antibody (ab89050)
  Anti-A1CF antibody (ab89050) at 1 µg/ml + Human placenta lysate at 50 µg
  
  Predicted band size : 65 kDa
  
• 

  Western blot - A1CF antibody (ab89050)
  Anti-A1CF antibody (ab89050) at 1 µg/ml + HeLa cell lysate at 50 µg
  
  Predicted band size : 65 kDa
  
• 

  Western blot - A1CF antibody (ab89050)
  All lanes : Anti-A1CF antibody (ab89050) at 1 µg/ml
  
  Lane 1 : A1CF-transfected 293T cell lysate
  Lane 2 : non transfected lysate
  
  Lysates/proteins at 25 µg per lane.
  
  
  Predicted band size : 65 kDa
  Observed band size : 65 kDa